Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
2nd Edition
ISBN: 9780134702339
Author: Erin C. Amerman
Publisher: PEARSON
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Chapter 20.6, Problem 1QC
How do innate immunity and adaptive immunity work together to defeat a cold virus?
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 20 Solutions
Human Anatomy & Physiology Plus Mastering A&P with Pearson eText -- Access Card Package (2nd Edition) (What's New in Anatomy & Physiology)
Ch. 20.1 - What are the main functions of the lymphatic...Ch. 20.1 - 2. How do lymphatic capillaries differ from blood...Ch. 20.1 - What is the pathway of lymph flow from the right...Ch. 20.1 - 4. What main cell types are located in lymphoid...Ch. 20.1 - List the main functions of MALT, lymph nodes, the...Ch. 20.2 - 1. How do innate immunity and adaptive immunity...Ch. 20.2 - 2. What are the three lines of defense?
Ch. 20.2 - What are the bodys main surface barriers, and how...Ch. 20.2 - Prob. 4QCCh. 20.2 - How are the immune system and lymphatic system...
Ch. 20.3 - Match the following cell types with their correct...Ch. 20.3 - Which of the cells in question 1 do not directly...Ch. 20.3 - What is the complement system, and what are its...Ch. 20.3 - In what two ways is the complement system...Ch. 20.3 - 5. What are the main cytokines of innate...Ch. 20.3 - What are the four cardinal signs of inflammation?Ch. 20.3 - 7. What are inflammatory mediators, and what do...Ch. 20.3 - Explain the role of phagocytes in the inflammatory...Ch. 20.3 - What causes the elevated temperature and common...Ch. 20.3 - Prob. 10QCCh. 20.4 - 1. What are MHC molecules?
Ch. 20.4 - 2. How do class I and class II MHC molecules...Ch. 20.4 - How are T cells activated?Ch. 20.4 - Prob. 4QCCh. 20.4 - What are the main functions of TC cells?Ch. 20.4 - Prob. 6QCCh. 20.4 - Prob. 7QCCh. 20.5 - 1. How is a B cell activated?
Ch. 20.5 - Prob. 2QCCh. 20.5 - What are the five classes of antibody, and how do...Ch. 20.5 - 4. List the five main functions of antibodies.
Ch. 20.5 - How do the primary and secondary immune responses...Ch. 20.5 - 6. What is the purpose of a vaccination, and what...Ch. 20.5 - 7. How do active immunity and passive immunity...Ch. 20.6 - How do innate immunity and adaptive immunity work...Ch. 20.6 - Walk through the basic steps of the immune...Ch. 20.6 - How does the immune system survey the body for...Ch. 20.6 - Walk through the steps of the immune response to...Ch. 20.6 - Prob. 5QCCh. 20.6 - 6. How do cancer cells escape the immune response...Ch. 20.7 - Define the four types of hypersensitivity...Ch. 20.7 - Explain how type IV hypersensitivity differs from...Ch. 20.7 - How do primary and secondary immunodeficiency...Ch. 20.7 - Prob. 4QCCh. 20.7 - What is an autoimmune disorder? What produces the...Ch. 20.7 - 6. What are the potential causes of autoimmune...Ch. 20 - 1. Which of the following is not a function of the...Ch. 20 - Mark the following statements as true or false. If...Ch. 20 - Fill in the blanks: The lymphoid organ that...Ch. 20 - Fill in the blanks: Nonspecific immunity is also...Ch. 20 -
5. Which of the following make up the body’s...Ch. 20 - Which of the following does not describe the...Ch. 20 - Mark the following statements as true or false. If...Ch. 20 - Which of the following functions is/are performed...Ch. 20 - Prob. 9CYRCh. 20 - 10. When naïve B cells are activated, they...Ch. 20 - 11. Match the following antibodies with the...Ch. 20 - Mark the following statements as true or false. If...Ch. 20 - Prob. 13CYRCh. 20 - On which type(s) of cells are class I MHC...Ch. 20 - Fill in the blanks: Class I MHC molecules display...Ch. 20 - 16. Which of the following is not a role of TH...Ch. 20 - 17. Mark the following statements as true or...Ch. 20 - 18. Type I hypersensitivity is due to release of...Ch. 20 - Prob. 19CYRCh. 20 - Prob. 20CYRCh. 20 - Prob. 1CYUCh. 20 - Complement proteins are crucial for stimulating...Ch. 20 - 3. Terrence has severe asthma and allergies, and...Ch. 20 - Your friend tests her snake-wrangling skills on an...Ch. 20 - 2. Carla presents to her physician with a...Ch. 20 - 3. Mr. White has been diagnosed with febrile...Ch. 20 - Mr. White developed neutropenia as a consequence...Ch. 20 - 5. The chemotherapeutic drugs Mr. White is taking...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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