Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 20.5, Problem 19ELO
Summary Introduction
To determine:
The diseases attributable to infection with species in the genus Haemophilus, and identify which is most severe.
Introduction:
Haemophilus is genus of encapsulated rods, that uses components released by lysed RBCs for their energy
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 20 Solutions
Foundations in Microbiology
Ch. 20.1 - Explain the effect of the virulence factor common...Ch. 20.1 - Identify those people most at risk of developing a...Ch. 20.1 - Briefly describe the human infections caused by...Ch. 20.1 - How can antibiotic treatment of a gram-negative...Ch. 20.2 - Name the genera of bacteria that are...Ch. 20.2 - Outline the pathology and epidemiology of...Ch. 20.2 - Explain the epidemiology of Francisella tularensis...Ch. 20.2 - Prob. 6ELOCh. 20.2 - Prob. 7ELOCh. 20.2 - Prob. 8ELO
Ch. 20.2 - List the four genera of bacteria that cause...Ch. 20.2 - Prob. 4CYPCh. 20.2 - Prob. 5CYPCh. 20.2 - Prob. 6CYPCh. 20.2 - What is unusual about the reservoir of Legionella?...Ch. 20.3 - Recall the medically important members of the...Ch. 20.3 - Prob. 10ELOCh. 20.3 - Explain the importance of the three major surface...Ch. 20.3 - Name the key characteristics shared by the...Ch. 20.3 - Explain what is meant by IMViC.Ch. 20.3 - Prob. 10CYPCh. 20.3 - Prob. 11CYPCh. 20.3 - Briefly describe the methods used to isolate and...Ch. 20.3 - Prob. 13CYPCh. 20.4 - Differentiate among the major enteric pathologies...Ch. 20.4 - Explain the role of E. coli in infantile and...Ch. 20.4 - Prob. 14ELOCh. 20.4 - Prob. 14CYPCh. 20.4 - Prob. 15CYPCh. 20.4 - Justify treating E. coli Ol57:H7 differently from...Ch. 20.4 - Prob. 17CYPCh. 20.5 - Differentiate between true noncoliform enteric...Ch. 20.5 - Distinguish the pathologies of typhoidal and...Ch. 20.5 - Identify the possible sources of Shigella...Ch. 20.5 - Prob. 18ELOCh. 20.5 - Prob. 19ELOCh. 20.5 - Prob. 18CYPCh. 20.5 - Prob. 19CYPCh. 20.5 - What are the Five F’s and how do they relate to...Ch. 20.5 - Prob. 22CYPCh. 20.5 - Which body systems are commonly infected by...Ch. 20.5 - Describe the epidemiology and pathology of...Ch. 20.L1 - A unique characteristic of many isolates of...Ch. 20.L1 - Prob. 2MCQCh. 20.L1 - Prob. 3MCQCh. 20.L1 - A classic symptom of pertussis is a. labored...Ch. 20.L1 - Prob. 5MCQCh. 20.L1 - Prob. 6MCQCh. 20.L1 - Prob. 7MCQCh. 20.L1 - Which of the following is a major difference...Ch. 20.L1 - A complication/complications of typhoid fever...Ch. 20.L1 - Prob. 10MCQCh. 20.L1 - Prob. 11MCQCh. 20.L1 - Haemophilus influnzae is ____________ and requires...Ch. 20.L1 - Prob. 13MCQCh. 20.L1 - Prob. 14MCQCh. 20.L1 - Single Matching. Match the infectious agent with...Ch. 20.L1 - Prob. 1CSRCh. 20.L1 - Prob. 2CSRCh. 20.L1 - Prob. 3CSRCh. 20.L1 - Prob. 1WCCh. 20.L1 - What are unique features in the epidemiology of E....Ch. 20.L1 - Explain several practices an individual can use to...Ch. 20.L1 - Prob. 4WCCh. 20.L1 - Briefly outline the zoonotic infections in this...Ch. 20.L2 - What is the logic behind testing for E. coli to...Ch. 20.L2 - Identify the genera with the following...Ch. 20.L2 - Given that so many infections arc caused by...Ch. 20.L2 - Prob. 4CTCh. 20.L2 - Students in our classes sometimes ask how it is...Ch. 20.L2 - Explain lhe reasons for an increase in numbers of...Ch. 20.L2 - Compare and contrast the pathology, diagnosis, and...Ch. 20.L2 - Prob. 8CTCh. 20.L2 - Prob. 9CTCh. 20.L2 - Prob. 10CTCh. 20.L2 - "There is no circumstance [in which] you can cook...Ch. 20.L2 - Use figure 20.5 a, b as a reference guideline for...
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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