Chapter 20.1, Problem 1CC

Summary Introduction

To determine: The bonds cleaved when restriction enzyme PvuI cuts its specific site in the DNA sequence.

Concept introduction:

Restriction enzymes recognize their specific target sequence and excise DNA at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.