The cloning of the human crystallin gene to obtain a sufficient amount of the human crystallin protein. Concept introduction : Protein encoding genes express themselves and form functional gene products in the form of proteins. This is known as gene expression. Generally, polymerase chain reaction (PCR) is used for the amplification of the gene sequence of interest and to synthesize its multiple copies. This gene fragment is then inserted into the expression vector to synthesize the protein encoded by the gene.

Question

Want to see the full answer?

Answer 1

Question

Chapter 20, Problem 9TYU

Summary Introduction

To explain: The cloning of the human crystallin gene to obtain a sufficient amount of the human crystallin protein.

Concept introduction:

Protein encoding genes express themselves and form functional gene products in the form of proteins. This is known as gene expression. Generally, polymerase chain reaction (PCR) is used for the amplification of the gene sequence of interest and to synthesize its multiple copies. This gene fragment is then inserted into the expression vector to synthesize the protein encoded by the gene.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Project: You want to make a cat that glows in the dark (its nose, ears, and tail should glow). Choose the best answer. 9) To get started on this project, you isolate and cut out the gene in a jellyfish that codes for the green fluorescent protein (GFP). The next thing you need to do is attach this gene to the correct promoter so that you can be sure it is expressed appropriately in the cat. Which of the following promoters should you use? a) any promoter is fine b) the original promoter found in the jellyfish c) a promoter from a gene that is expressed in cells of the cat's nose, ears, and tail 10) You are now ready to transfer the piece of recombinant DNA you have prepared to the cat. You want to be sure that the transgenic cat you create will be able to pass the fluorescence on to its offspring. What is the best type of cell to transfer the recombinant DNA to? a) an unfertilized egg b) a fertilized egg c) somatic cells of an adult cat

Pls help ASAP

Now you have the gene sequence. Now you would like to clone it into an expression vector to grow up in a bacterial system. Because you're going to use bacteria to generate protein from a eukaryote, the mammoth, you need to get rid of introns from your sequence. How do you do that? Bioinformatically, I look for splice-site sequences and branch-point adenines and predict intron-exon boundaries I use a comparative genomic approach and use sequence homology with the genome of a closely related species I use a comparative genomic approach and use sequence homology with the genome of a distantly related species Both A and B Both B and C Why did you bother to identify the introns? So that I could include them in the sequence to understand intron function. So that I could exclude them from the sequence because prokaryotes don't have spliceosomal machinery. So that I could see how introns affect protein folding.