BIOCHEMISTRY (LL)
9th Edition
ISBN: 9781337805100
Author: Campbell
Publisher: CENGAGE L
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Chapter 20, Problem 49RE
MATHEMATICAL What yield of ATP can be expected from complete oxidation of each of the following substrates by the reactions of glycolysis, the citric acid cycle, and oxidative phosphorylation?
(a) Fructose-1,6-bisphosphate
(b) Glucose
(c) Phosphoenolpyruvate
(d) Glyceraldehyde-3-phosphate
(e) NADH
(f) Pyruvate
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In 1956, E. P. Kennedy and S. B. Weiss published their study of membrane lipid phosphatidylcholine (lecithin) synthesis in rat
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incubating 32 P-labeled phosphocholine at physiological temperature (37 °C) with broken cells from rat liver yielded labeled
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Determine the optimal pH for this enzyme and characterize the enzyme activity at different pH values.
-O-P-O-CH2-CH₁₂-N(CH3)3
Phosphocholine
H₂C-O-C-R
HC-O-C-R2
+
+
+
Cell fraction + ?
HC-O-P-O-CH₁₂-CH₂-N(CH),
O
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The researchers then centrifuged the broken cell preparation
to separate the membranes from the soluble proteins. They
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soluble proteins. Table 1 summarizes the results.
Table 1: Cell fraction requirement for incorporation of
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Researchers isolated an unknown substance, X, from rabbit muscle. They determined its structure from the following
observations and experiments.
(a) Qualitative analysis showed that X was composed entirely of C, H, and O. A weighed sample of X was completely oxidized
and the H2O and CO2 produced were measured. This quantitative analysis revealed that X contained 40.00% C, 6.71% H, and
53.29% O by weight.
(b) The molecular mass of X, as determined by mass spectrometry, was 90.00 atomic mass units (u).
(c) Infrared spectroscopy showed that X contained one double bond.
(d) X dissolved readily in water, and the solution demonstrated optical activity when tested in a polarimeter.
(e) The aqueous solution of X is acidic.
What is the empirical formula of X?
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Chapter 20 Solutions
BIOCHEMISTRY (LL)
Ch. 20 - RECALL Briefly summarize the steps in the electron...Ch. 20 - RECALL Are electron transport and oxidative...Ch. 20 - Prob. 3RECh. 20 - REFLECT AND APPLY Show how the reactions of the...Ch. 20 - REFLECT AND APPLY How does mitochondrial structure...Ch. 20 - Prob. 6RECh. 20 - Prob. 7RECh. 20 - MATHEMATICAL Using the information in Table 20.2,...Ch. 20 - Prob. 9RECh. 20 - Prob. 10RE
Ch. 20 - MATHEMATICAL Calculate E for the following...Ch. 20 - Prob. 12RECh. 20 - MATHEMATICAL Which is more favorable...Ch. 20 - REFLECT AND APPLY Comment on the fact that the...Ch. 20 - RECALL What do cytochromes have in common with...Ch. 20 - RECALL How do the cytochromes differ from...Ch. 20 - RECALL Which of the following does not play a role...Ch. 20 - Prob. 18RECh. 20 - Prob. 19RECh. 20 - REFLECT AND APPLY Two biochemistry students are...Ch. 20 - REFLECT AND APPLY Cytochrome oxidase and...Ch. 20 - Prob. 22RECh. 20 - REFLECT AND APPLY Reflect on the evolutionary...Ch. 20 - REFLECT AND APPLY Experimental evidence strongly...Ch. 20 - Prob. 25RECh. 20 - REFLECT AND APPLY What is the advantage of having...Ch. 20 - REFLECT AND APPLY Why do the electron-transfer...Ch. 20 - Prob. 28RECh. 20 - Prob. 29RECh. 20 - Prob. 30RECh. 20 - RECALL Describe the role of the F1 portion of ATP...Ch. 20 - Prob. 32RECh. 20 - Prob. 33RECh. 20 - Prob. 34RECh. 20 - REFLECT AND APPLY What is the approximate P/O...Ch. 20 - REFLECT AND APPLY Why is it difficult to determine...Ch. 20 - REFLECT AND APPLY What are some of the...Ch. 20 - RECALL Briefly summarize the main arguments of the...Ch. 20 - Prob. 39RECh. 20 - Prob. 40RECh. 20 - Prob. 41RECh. 20 - Prob. 42RECh. 20 - REFLECT AND APPLY Criticize the following...Ch. 20 - Prob. 44RECh. 20 - Prob. 45RECh. 20 - Prob. 46RECh. 20 - RECALL How does the yield of ATP from complete...Ch. 20 - REFLECT AND APPLY The malate-aspartate shuttle...Ch. 20 - MATHEMATICAL What yield of ATP can be expected...Ch. 20 - MATHEMATICAL The free-energy change (G) for the...
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