Chapter 20, Problem 20.96QP

Interpretation Introduction

Interpretation:

DNA sequencing gel that would represent the following sequence has to be drawn.

  ${\text{5}}^{\text{'}}{\text{-GACTATCCTAG-3}}^{\text{'}}$

Concept Introduction:

In Sanger’s method of DNA sequencing, a cloned piece of DNA is separated into two strands. Each of these serves as a template strand. To the four test tubes the DNA polymerases, a primer, deoxynucleotide triphosphate and dideoxynucleotide analog corresponding to each nucleotide are added.

Replication occurs but as soon as the dideoxynucleotide analog of the nucleotides is confronted the replication stops. But since the concentration of dideoxynucleotide is low replication halts at specific locations and thus yields fragments of different sizes. The fragments are then analyzed by gel electrophoresis.