Chapter 20, Problem 20.95QP

Interpretation Introduction

Interpretation:

The location of DNA pieces given in the following sequences has to be mapped.

$\begin{array}{l}\text{(a) : }5\prime \text{-AGCTCCTGATTTCATACAGTTTCTACTACCTACTA-}3\text{'}\\ \text{(b) : 5'-AGACATTCTATCTACCTAGACTATGTTCAGAA-3'}\\ \text{(c) : }5\prime \text{-TTCAGAACTCATTCAGACCTACTACTATACCTTGGGAGCTCCT-}3\prime \\ (\text{d}):5\prime \text{-ACCTACTAGACTATACTACTACTAAGGGGACTATTCCAGACTT-}3\prime \end{array}$

Concept Introduction:

There are two strategies to analyze the entire genome. One is the creation of the genomic library and chromosome walking. The method of chromosome walking uses overlapping genes so that the entire sequence is cloned. This enables the sequences to be mapped.