CAMPBELL BIOLOGY (18W)
CAMPBELL BIOLOGY (18W)
12th Edition
ISBN: 9780136858256
Author: Urry
Publisher: PEARSON
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Chapter 20, Problem 20.2CR

What useful Information is obtained by detecting expression ot specific genes?

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A complex biochemical pathway is shown below, along with the alleles that either promote or inhibit each step of the pathway leading to a phenotype. Gene A has alleles A and a, B has alleles B and b, and so forth. Genes B and C are duplicate dominant epistatic lethal as heterozygotes (i.e. Bb Cc are lethal). Genes D and E are duplicate dominant epistatic (i.e. dd eg = desired phenotype). If I were to cross AA Bb cc Dd Ee with aa BB Cc Dd e, (i) (ii) What proportion of all offspring don't show the phenotype? What proportion of offspring survive? Gene A Gene B B Gene D a Gene C Gene E
The expression levels for two genes measured at four times are: Compute the correlation coefficient for this pair of genes. Make a plot of the gene1 levelsagainstthe gene 2levels.Isthe informationinthe plot consistent with the implication of the correlation coefficient? Comment on potential hazards of using the correlation coefficient based on your findings.  
When performing QPCR to study differential gene expression, what is the role of SYBR green? What is the most correct answer? SYBRgreen intercalates proportionally and efficiently with double stranded DNA and can therefore be used to quantify it   SYBRgreen intercalates proportionally and efficiently with nucleic acids and can therefore be used to quantify them.   SYBRgreen intercalates more efficiently and proportionately with single stranded DNA and can therefore be used to quantify cDNA (a direct product of mRNA).   SYBRgreen intercalates proportionally efficiently with messenger RNA and can therefore be used to quantify it.
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