Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 1CT
The following graph shows a likely relationship between bacterial growth and oxygen use in glucose broth Explain the relationship.
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Define the term substrate-level phosphorylation:How does it differ from oxidative phosphorylation?What compound(s) do fermentative bacteria need tosynthesize in order to make ATP by substrate-levelphosphorylation?
Yeast cells are added to a 3.0 L batch bioreactor so that the initial cell concentration is [X]. = 1.3 g cells / L.
The growth mediğim in the reactor, which is well-mixed so the cells have access to all nutrients, contains 150 g
CELL DATA
ribose (C5H10O5, MW 150), 75 g ammonia (NH3, MW 17), and 85 g
oxygen (O2, MW 32).
A-10. Determine the maximum concentration of cells, [X], that will form in the
bioreactor when the limiting nutrient is consumed.
Search
Yx/ribose
YX/02
YX/NH3
Specific growth rate
on limiting nutrient, u
Lag phase duration
Deceleration phase
duration
0.48 g/g
0.87 g/g
0.23 g/g
0.51 h
30 minutes
Negligible
In aerobic respiration, cells generate heat as they grow. The amount of heat generated by cells
is proportional to the amount of oxygen they consume. Accordingly, it was found that 460 kJ is generated
per gmol of O2 consumed.
Suppose that you need to choose a fermenter for optimal growth of Bacillus subtilis cells. It is known that
the maximum oxygen demand of B. subtilis is 100 mol m3 h1. A vendor offers you a reactor with a
cylindrical shape (2.8 m in diameter) and 15 m³ volume. The reactor includes a cooling coil with 30 m
length and a pipe diameter of 6 cm, through which. For practical purposes, power input by the rotating
impeller inside the bioreactor can be neglected.
Does this reactor meet the cooling requirements of your production process?
Note: If you think you need more information than you are provided with here to solve this problem, use
online resources to find the required information. In this case, please state your source clearly.
Note: You may assume that the…
Chapter 20 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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