BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
10th Edition
ISBN: 9781305967359
Author: STARR
Publisher: CENGAGE L
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Chapter 20, Problem 13SA
Summary Introduction
Introduction:
The Euglena is a genus that consists of single cell flagellate eukaryotes. These are found in marine and freshwater. They are differentiated on the basis of the presence of cell covering which is known as a pellicle. Euglena has similar characters to both plants and animals.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 20 Solutions
BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
Ch. 20 - All protists _______. a. lack mitochondria c. live...Ch. 20 - Deposits of shells from ancient ________ have been...Ch. 20 - The presence of a contractile vacuole indicates...Ch. 20 - Prob. 4SACh. 20 - Prob. 5SACh. 20 - Prob. 6SACh. 20 - Prob. 7SACh. 20 - Some _______ are bioluminescent and other live...Ch. 20 - Prob. 9SACh. 20 - A high concentration of oil helps _______ stay...
Ch. 20 - Prob. 11SACh. 20 - Prob. 12SACh. 20 - Prob. 13SACh. 20 - Prob. 14SACh. 20 - Prob. 15SACh. 20 - Imagine you are in a developing country where...Ch. 20 - Which groups of protists are scientists most...Ch. 20 - Water in abandoned swimming pools often turns...Ch. 20 - Melvin Calvin and Andrew Benson determined the...Ch. 20 - The protist that causes malaria evolved from a...
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