Biology Now
Biology Now
1st Edition
ISBN: 9780393918922
Author: Anne Houtman, Megan Scudellari, Cindy Malone, Anu Singh-Cundy
Publisher: W. W. Norton & Company
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Chapter 2, Problem 8TQ
Summary Introduction

If a molecule consists of atoms from two or more dissimilar elements is known as a chemical compound. Carbon atoms can bond with one another and with other atoms in order to develop an abundant diversity of chemical compounds, which is known as organic molecules. There are four types of organic molecules, which are carbohydrates, proteins, lipids, and nucleic acids. These organic molecules or biomolecules are crucial for living cells.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY