EBK CAMPBELL BIOLOGY
10th Edition
ISBN: 9780136539414
Author: Reece
Publisher: VST
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Textbook Question
Chapter 2, Problem 4TYU
Which Statement is true of all atoms that are anions?
- (A) The atom has more electrons than protons.
- (B) The atom has more protons than electrons.
- (C) The atom has fewer protons than does a neutral atom of the same element.
- (D) The atom has more neutrons than protons.
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a) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein?
b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control.
c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
Chapter 2 Solutions
EBK CAMPBELL BIOLOGY
Ch. 2.1 - MAKE CONNECTIONS Explain how table salt has...Ch. 2.1 - Is a trace element an essential element? Explain.Ch. 2.1 - Prob. 3CCCh. 2.1 - MAKE CONNECTIONS Explain how natural selection...Ch. 2.2 - Prob. 1CCCh. 2.2 - A nitrogen atom has 7 protons, and the most common...Ch. 2.2 - Prob. 3CCCh. 2.2 - Prob. 4CCCh. 2.3 - Why does the structure H C = C H fail to make...Ch. 2.3 - What holds the atoms together in a crystal of...
Ch. 2.3 - What holds the atoms together in a crystal of...Ch. 2.4 - Prob. 1CCCh. 2.4 - Which type of chemical reaction, if any, occurs...Ch. 2.4 - WHAT IF? Write an equation that uses the products...Ch. 2 - Prob. 2.1CRCh. 2 - DRAW IT Draw the electron distribution diagrams...Ch. 2 - In terms of electron sharing between atoms,...Ch. 2 - What would happen to the concentration of products...Ch. 2 - Level 1: Knowledge/Comprehension 1. In the term...Ch. 2 - Compared with 31P, the radioactive isotope 32P has...Ch. 2 - The reactivity of an atom arises from (A) the...Ch. 2 - Which Statement is true of all atoms that are...Ch. 2 - Which of the following statements correctly...Ch. 2 - Prob. 6TYUCh. 2 - The atomic number of sulfur is 16. Sulfur combines...Ch. 2 - What coefficients must be placed in the following...Ch. 2 - DRAW IT Draw Lewis dot structures for each...Ch. 2 - EVOLUTION CONNECTION The percentages of naturally...Ch. 2 - Prob. 11TYUCh. 2 - Prob. 12TYUCh. 2 - Prob. 13TYU
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
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