Nester's Microbiology: A Human Perspective
8th Edition
ISBN: 9780073522593
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 2, Problem 4SA
Summary Introduction
To review:
The significance of cellulose.
Introduction:
Cellulose is a type of structural polysaccharide. It is mostly found in the cell walls of plants, inballs of cotton produced by the cotton plant, and comprises around 50% of the dryweight of plant trunks. Plants produce up to a trillion tons of cellulose. Thus, it is the mostabundant organic molecule found on earth. Cellulose is not digested by humans becauseof the absence of cellulase enzyme.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 2 Solutions
Nester's Microbiology: A Human Perspective
Ch. 2 - Differentiate between an atom, a molecule, and a...Ch. 2 -
2. Why is water a good solvent?
Ch. 2 - Which solution is more acidic, one with a pH of 4...Ch. 2 - Prob. 4SACh. 2 - Prob. 5SACh. 2 - What is a structural isomer?Ch. 2 - Name the major groups of lipids and give an...Ch. 2 - List six functions of proteins.Ch. 2 -
9. What are the four levels of protein structure,...Ch. 2 - How do DNA and RNA differ from one another in...
Ch. 2 - Choose the list that goes from the lightest to the...Ch. 2 -
2. An oxygen atom has an atomic number of 8. It...Ch. 2 - Dehydration synthesis is involved in the synthesis...Ch. 2 - The primary structure of a protein relates to its...Ch. 2 -
5. Pure water has all of the following properties...Ch. 2 - When the pH of a solution changes from 3 to 8, the...Ch. 2 - A shortage of nitrogen (N) would make it most...Ch. 2 - Complementarity plays a major role in the...Ch. 2 - Prob. 9MCCh. 2 - The function of a buffer is to a) bring the pH of...Ch. 2 - A group of prokaryotes known as thermophiles...Ch. 2 -
2. Microorganisms use hydrogen bonds to attach to...Ch. 2 - What properties of the carbon atom make it ideal...Ch. 2 -
2. A biologist determined the amounts of several...Ch. 2 - The table on the right indicates the freezing and...
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