Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 2, Problem 27P
Summary Introduction

To determine: The preparation of 1.0 L of 0.10 M acetate buffer of pH 4.00 given that 0.10M solution of acetic acid (pKa 4.76) and sodium acetate.

Introduction:

The Henderson-Hasselbalch equation represents the measurement of acidity as pH (potential of hydrogen) in biochemical reactions. The isoelectric point and pH of buffers are calculated by this reaction. The pH is used to calculate the pKa of molecules. The “ionized and unionized” concentrations of chemicals are estimated with the help of this equation. The pKa is used to calculate the pH of molecules.

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