Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 2, Problem 18P
Summary Introduction

To determine: The pH of a solution containing 0.12 mol/L of NH4Cl and 0.03 mol/L of NaOH ( pKa of NH4+/NH3 is 9.25 ) is to be determined.

Introduction:

The Henderson-Hasselbalch equation depicts the measurement of acidity as pH (potential of hydrogen) in biochemical reactions. The isoelectric point and pH of buffers are calculated by this reaction. The pH is used to calculate the pKa of molecules. The “ionized and unionized” concentrations of chemicals are estimated with the help of this equation. The pKa is used to calculate the pH of molecules.

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