(a)
Interpretation:
The performance of chloroplast ATP synthase in the presence of DDT alone and DDT+ with thioredoxin present should be monitored.
Concept introduction:
DDT stands for Dichlorodiphenyltrichloroethane. This prevents the complete repolarization of mitochondria. High concentrations of DDT leads to the destruction of the structural integrity of mitochondria.
Thioredoxin is a class of small redox proteins, which acts as a redox signal in organisms.
(b)
Interpretation:
The graphical behavior of control enzyme and modified enzyme when DDT concentration increased along the x-axis should be monitored.
Concept introduction:
DDT which stands for Dithiothreitol is a reducing agent which is found in biological systems.
(c)
Interpretation:
The graphical behavior of modified enzyme which thioredoxin present and absent should be observed.
Concept introduction:
Thioredoxin & DDT are reducing agents known to be present in
(d)
Interpretation:
Overall four graphs should be monitored.
Concept introduction:
This experiment is planned to determine the responsible sub unit for the difference in ATPase activity in Chloroplast & Mitochondria.
(e)
Interpretation:
The biological process limited only to plants should be considered.
Concept introduction:
The biological process which is unique to plants is photosynthesis. It is the process of converting of the energy in sun light to chemical energy.
(f)
Interpretation:
For this part twenty amino acids with its structures should be considered.
Concept introduction:
Amino acid is the primary unit of protein. It has four groups as carbonyl group (- COOH),
(g)
Interpretation:
Experiments related to prove reducing ability of cysteine should be mentioned.
Concept introduction:
Reducing agents has the ability to reduce. It is the capability to capture electrons in the medium.
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Chapter 19 Solutions
BIOCHEMISTRY
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BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning