LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
4th Edition
ISBN: 9781264929290
Author: McKinley
Publisher: MCG CUSTOM
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Textbook Question
Chapter 19, Problem 20DYKB
Define cardiac output, and explain how it is influenced by both heart rate and stroke volume.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 19 Solutions
LSC ANATOMY & PHYSIOLOGY CONNECT ACCESS
Ch. 19.1 - Prob. 1WDYLCh. 19.1 - Prob. 2WDYLCh. 19.1 - Prob. 3WDYLCh. 19.1 - Prob. 4WDYLCh. 19.1 - Prob. 5WDYLCh. 19.2 - What is the bony structure that protects both the...Ch. 19.2 - Prob. 7WDYLCh. 19.2 - Prob. 8WDYLCh. 19.3 - Prob. 9WDYLCh. 19.3 - Prob. 10WDYL
Ch. 19.3 - Prob. 11WDYLCh. 19.3 - Prob. 12WDYLCh. 19.3 - Prob. 13WDYLCh. 19.3 - Prob. 14WDYLCh. 19.3 - Prob. 15WDYLCh. 19.3 - Prob. 16WDYLCh. 19.3 - What areas of the heart are deprived of blood when...Ch. 19.3 - Prob. 18WDYLCh. 19.4 - Prob. 19WDYLCh. 19.5 - Prob. 20WDYLCh. 19.5 - Which autonomic division is associated with the...Ch. 19.6 - Prob. 22WDYLCh. 19.6 - What is autorhythmicity? Describe how nodal cells...Ch. 19.6 - What is the path of an action potential through...Ch. 19.6 - What anatomic features slow the conduction rate of...Ch. 19.7 - In which direction does Ca2+ move in response to...Ch. 19.7 - What three electrical events occur at the...Ch. 19.7 - What is the significance of the extended...Ch. 19.7 - What events in the heart are indicated by each of...Ch. 19.8 - Pressure changes that occur during the cardiac...Ch. 19.8 - What is occurring during ventricular ejection?Ch. 19.8 - Prob. 32WDYLCh. 19.8 - Define end-diastolic volume, end-systolic volume,...Ch. 19.9 - What are the two factors that determine cardiac...Ch. 19.9 - What is the cardiac output at rest and during...Ch. 19.9 - Prob. 36WDYLCh. 19.9 - Describe the atrial reflex, which involves...Ch. 19.9 - Prob. 38WDYLCh. 19.9 - Prob. 39WDYLCh. 19.10 - What would be the path of blood flow through the...Ch. 19 - Which of the following is the correct circulatory...Ch. 19 - The pericardial cavity is located between the a....Ch. 19 - How is blood prevented from backflowing from the...Ch. 19 - ____ 4. Venous blood draining from the heart wall...Ch. 19 - _____ 5. Calcium channels in the nodal cells...Ch. 19 - ____6. Action potentials are spread rapidly...Ch. 19 - Why is it necessary to stimulate papillary muscles...Ch. 19 - ____ 8. Preload is a measure of a. stretch of...Ch. 19 - ____ 9. All of the following occur when the...Ch. 19 - ____10. What occurs during the atrial reflex? a....Ch. 19 - Prob. 11DYKBCh. 19 - Compare the structure, location, and function of...Ch. 19 - Prob. 13DYKBCh. 19 - Explain why the walls of the atria are thinner...Ch. 19 - Describe the structure and function of...Ch. 19 - Explain the general location and function of...Ch. 19 - Describe the functional differences in the effects...Ch. 19 - Prob. 18DYKBCh. 19 - List the five events of the cardiac cycle, and...Ch. 19 - Define cardiac output, and explain how it is...Ch. 19 - A young man was doing some vigorous exercise when...Ch. 19 - A young man was doing some vigorous exercise when...Ch. 19 - Prob. 3CALCh. 19 - Prob. 4CALCh. 19 - During surgery, the right vagus nerve was...Ch. 19 - Prob. 1CSLCh. 19 - Prob. 2CSLCh. 19 - Your grandfather was told that his SA node...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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