Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 19, Problem 1VI
Summary Introduction
To determine:
The genera of pathogens that appeared in the particular stained smears.
Introduction:
Staining is a technique that is used to visualize microorganisms so that there could be an enhanced contrast to distinguish the microscopic image. Various dyes and stains are used in staining. These include acid fast stain, methenamine silver, safranin, gentian violet, and many more.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 19 Solutions
Microbiology with Diseases by Taxonomy (5th Edition)
Ch. 19 - Prob. 1MCCh. 19 - Prob. 2MCCh. 19 - Prob. 3MCCh. 19 - Prob. 4MCCh. 19 - Prob. 5MCCh. 19 - Prob. 6MCCh. 19 - Prob. 7MCCh. 19 - Prob. 8MCCh. 19 - Prob. 9MCCh. 19 - Prob. 10MC
Ch. 19 - Which of the following is not characteristic of...Ch. 19 - Prob. 1MCh. 19 - Prob. 1SACh. 19 - Prob. 2SACh. 19 - Contrast tuberculoid leprosy with lepromatous...Ch. 19 - Prob. 4SACh. 19 - Why do pediatricians recommend that children under...Ch. 19 - Prob. 6SACh. 19 - Prob. 7SACh. 19 - Prob. 8SACh. 19 - Prob. 9SACh. 19 - Prob. 10SACh. 19 - Prob. 11SACh. 19 - Prob. 12SACh. 19 - Prob. 1VICh. 19 - Prob. 2VICh. 19 - A few days after the death of a child hospitalized...Ch. 19 - Prob. 2CTCh. 19 - Prob. 3CTCh. 19 - Prob. 4CTCh. 19 - Prob. 5CTCh. 19 - Prob. 6CTCh. 19 - Prob. 1CMCh. 19 - Prob. 1TMWCh. 19 - In 1928, Frederick Griffith discovered genetic...Ch. 19 - Prob. 3TMWCh. 19 - Prob. 4TMWCh. 19 - Why does use of broad-spectrum antibacterial drugs...Ch. 19 - Prob. 6TMWCh. 19 - Why do pediatricians refrain from using...Ch. 19 - Why is it necessary to get a diphtheria...Ch. 19 - Prob. 9TMWCh. 19 - Prob. 10TMWCh. 19 - Why does Actinomyces appear purple with a Gram...Ch. 19 - Clinical Case Study A Fatal Case of...Ch. 19 - Prob. 2CCSCh. 19 - Prob. 1EDCS
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