EBK EXPLORING CHEMICAL ANALYSIS
5th Edition
ISBN: 8220101443908
Author: Harris
Publisher: MAC HIGHER
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Chapter 19, Problem 19.21P
Interpretation Introduction
Interpretation:
Amplification of analyte response as done in enzyme-linked immunoassay technique has to be explained.
Concept Introduction:
Immunoassay is important biochemical tool that can measure and quantify the concentration of small molecule. Immunoassay is often supported by antibodies called antigens. ELISA is one such enzyme-linked immunoassay technique.
In ELISA technique certain target macromolecule called antigen is immobilized and fixed to surface of microplate. This then forms complex with reporter enzyme and detection is accomplished by measurement of reporter enzyme’s activity via incubation to produce significant product. Essential feature of this method is extremely specific antigen-antibody interaction.
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6. Consider the following exothermic reaction below.
2Cu2+(aq) +41 (aq)2Cul(s) + 12(aq)
a. If Cul is added, there will be a shift left/shift right/no shift (circle one).
b. If Cu2+ is added, there will be a shift left/shift right/no shift (circle one).
c. If a solution of AgNO3 is added, there will be a shift left/shift right/no shift (circle one).
d. If the solvent hexane (C6H14) is added, there will be a shift left/shift right/no shift (circle
one). Hint: one of the reaction species is more soluble in hexane than in water.
e. If the reaction is cooled, there will be a shift left/shift right/no shift (circle one).
f. Which of the changes above will change the equilibrium constant, K?
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EBK EXPLORING CHEMICAL ANALYSIS
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