Amplification of analyte response as done in enzyme-linked immunoassay technique has to be explained. Concept Introduction: Immunoassay is important biochemical tool that can measure and quantify the concentration of small molecule. Immunoassay is often supported by antibodies called antigens. ELISA is one such enzyme-linked immunoassay technique. In ELISA technique certain target macromolecule called antigen is immobilized and fixed to surface of microplate. This then forms complex with reporter enzyme and detection is accomplished by measurement of reporter enzyme’s activity via incubation to produce significant product. Essential feature of this method is extremely specific antigen-antibody interaction.

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Chapter 19, Problem 19.21P

Interpretation Introduction

Interpretation:

Amplification of analyte response as done in enzyme-linked immunoassay technique has to be explained.

Concept Introduction:

Immunoassay is important biochemical tool that can measure and quantify the concentration of small molecule. Immunoassay is often supported by antibodies called antigens. ELISA is one such enzyme-linked immunoassay technique.

In ELISA technique certain target macromolecule called antigen is immobilized and fixed to surface of microplate. This then forms complex with reporter enzyme and detection is accomplished by measurement of reporter enzyme’s activity via incubation to produce significant product. Essential feature of this method is extremely specific antigen-antibody interaction.

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