Foundations in Microbiology
9th Edition
ISBN: 9780073522609
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 18.L1, Problem 1WC
Summary Introduction
To determine:
The actions of hemolysins, leukocidin, kinases, hyaluronidase, and coagulase that make them virulence factors.
Introduction:
Virulence factors are the
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 18 Solutions
Foundations in Microbiology
Ch. 18.1 - Prob. 1ELOCh. 18.1 - Describe the ability of S. aureus to withstand...Ch. 18.1 - Prob. 3ELOCh. 18.1 - Summarize the cutaneous, systemic, and toxigenic...Ch. 18.1 - Prob. 5ELOCh. 18.1 - Explain strategies used to prevent and treat...Ch. 18.1 - Prob. 1CYPCh. 18.1 - Discuss why food intoxication in salty foods, such...Ch. 18.1 - Explain how unbroken skin, the phagocytic action...Ch. 18.1 - List the strategies commonly used to prevent and...
Ch. 18.1 - Explain the meanings of the acronyms MRSA, VRE,...Ch. 18.2 - Name the most important human pathogens in the...Ch. 18.2 - Summarize the virulence factors of S. pyogenes, as...Ch. 18.2 - Prob. 9ELOCh. 18.2 - Explain strategies used to prevent and treat...Ch. 18.2 - Relate the pathogenesis and epidemiology of S....Ch. 18.2 - List the clinically significant pathogens in the...Ch. 18.2 - Describe the immediate effects and long-term...Ch. 18.2 - Prob. 8CYPCh. 18.2 - Differentiate between pneumococcal disease...Ch. 18.3 - Name the most important human pathogens in the...Ch. 18.3 - Compare the differences in pathologies of N....Ch. 18.3 - Summarize the pathogenesis and epidemiology of N....Ch. 18.3 - Recall the medically important genera, besides...Ch. 18.3 - Describe the epidemiology and pathology of N....Ch. 18.3 - List the short-term and long-term consequences of...Ch. 18.3 - Prob. 12CYPCh. 18.L1 - Which of the following is/are a pyogenic...Ch. 18.L1 - The coagulase text is used primarily to...Ch. 18.L1 - Prob. 3MCQCh. 18.L1 - The most severe streptococcal diseases are caused...Ch. 18.L1 - 5. Rheumatic fever damages the _______, and...Ch. 18.L1 - ______ hemolysis is the partial lysis of red blood...Ch. 18.L1 - Prob. 7MCQCh. 18.L1 - Which of the following strains of pathogens has...Ch. 18.L1 - Otitis media is a/an _______ infection often...Ch. 18.L1 - Prob. 10MCQCh. 18.L1 - Prob. 11MCQCh. 18.L1 - Prob. 12MCQCh. 18.L1 - Prob. 13MCQCh. 18.L1 - Which infectious agent of those covered in the...Ch. 18.L1 - Prob. 15MCQCh. 18.L1 - Prob. 16MCQCh. 18.L1 - The first step in the clinical diagnosis of...Ch. 18.L1 - Vaccination can provide protection against N....Ch. 18.L1 - In this case, the first step taken to treat...Ch. 18.L1 - Prob. 1WCCh. 18.L1 - What conditions favor staph food poisoning?Ch. 18.L1 - Prob. 3WCCh. 18.L1 - Prob. 4WCCh. 18.L1 - Discuss the apparent pathology at work in...Ch. 18.L1 - Prob. 6WCCh. 18.L1 - a. Compare and contrast the characteristics of...Ch. 18.L2 - You have been handed the problem of diagnosing...Ch. 18.L2 - You have been called upon to prevent outbreaks of...Ch. 18.L2 - Prob. 3CTCh. 18.L2 - Prob. 4CTCh. 18.L2 - Prob. 5CTCh. 18.L2 - Prob. 6CTCh. 18.L2 - Prob. 1VCCh. 18.L2 - Prob. 2VC
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
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