ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Question
Chapter 18.4, Problem 18WDYL
Summary Introduction
To determine:
That what prevents platelets from forming plugs in healthy blood vessels.
Concept introduction:
Platelets, which are also called as thrombocytes are the membrane-bound cell fragments. They do not have a nucleus and are not considered as true cells. The main function of platelets is maintaining the hemostasis. It is a process by which bleeding was stopped by clotting of blood at the time of injury or cut.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 18 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 18.1 - Prob. 1WDYLCh. 18.1 - How does blood help regulate body temperature and...Ch. 18.1 - Will blood be able to properly carry out its...Ch. 18.1 - Prob. 4WDYLCh. 18.1 - Prob. 5WDYLCh. 18.2 - Prob. 6WDYLCh. 18.2 - Prob. 7WDYLCh. 18.2 - Prob. 8WDYLCh. 18.3 - What are the two main types of precursor cells for...Ch. 18.3 - Prob. 10WDYL
Ch. 18.3 - Prob. 11WDYLCh. 18.3 - Prob. 12WDYLCh. 18.3 - Prob. 13WDYLCh. 18.3 - Prob. 14WDYLCh. 18.3 - Prob. 15WDYLCh. 18.3 - Describe the process of erythropoiesis, beginning...Ch. 18.4 - Prob. 17WDYLCh. 18.4 - Prob. 18WDYLCh. 18.4 - Prob. 19WDYLCh. 18.4 - Prob. 20WDYLCh. 18.4 - At what point in blood loss is the sympathetic...Ch. 18.4 - Prob. 22WDYLCh. 18.5 - Prob. 23WDYLCh. 18.5 - Prob. 24WDYLCh. 18 - Prob. 1DYKBCh. 18 - _____ 2. Which type of leukocyte increases during...Ch. 18 - Prob. 3DYKBCh. 18 - Prob. 4DYKBCh. 18 - Prob. 5DYKBCh. 18 - Prob. 6DYKBCh. 18 - Prob. 7DYKBCh. 18 - _____ 8. During the recycling of components...Ch. 18 - _____ 9. The extrinsic pathway of coagulation is...Ch. 18 - Prob. 10DYKBCh. 18 - How does blood help regulate body temperature?Ch. 18 - What are alpha- and beta-globulins? What do they...Ch. 18 - When blood is centrifuged, a thin, whitish-gray...Ch. 18 - What is the shape of an erythrocyte, and why is...Ch. 18 - How are respiratory gases (oxygen and carbon...Ch. 18 - What are the anatomic characteristics of each type...Ch. 18 - How do the functions of basophils differ from...Ch. 18 - Briefly describe the origin, structure, and...Ch. 18 - Prob. 19DYKBCh. 18 - Describe the three phases of hemostasis, and list...Ch. 18 - Use the following paragraph to answer questions...Ch. 18 - Prob. 2CALCh. 18 - Which sequence or pathway best describes the...Ch. 18 - Prob. 4CALCh. 18 - Prob. 5CALCh. 18 - While taking a clinical laboratory class, Marilyn...Ch. 18 - Abby is a nurse on duty in a hospital emergency...Ch. 18 - Prob. 3CSL
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