Genetics
Genetics
5th Edition
ISBN: 9781464109461
Author: Benjamin A. Pierce
Publisher: MAC HIGHER
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Chapter 18.1, Problem 45CQ

a.

Summary Introduction

To determine:

R1 and SuB mutations are intragenic suppressors or intergenic suppressors.

Introduction:

Person B and Person C conducted nonsense mutation on E. coli strain. During mutation, the structural gene encodes an enzyme called L-ribulokinase that allows the bacteria to metabolize arabinose sugar. The revertants of mutant strain showed two different suppressor mutation R1 and SuB. R1is the original mutation in L-ribulokinase and not able to metabolize arabinose sugar as the enzyme encoded by the wild type allele. The second suppressor mutation SuB is not linked to the original mutation. However, the second suppressor able to produce L-ribulokinase in conjunction with R1 mutation

b.

Summary Introduction

To explain:

The way in which R1 and SuB restore the ability of araB14 to metabolize arabinose and why SuB is able to more fully restore this ability.

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