EBK MICROBIOLOGY
12th Edition
ISBN: 8220100659720
Author: CASE
Publisher: PEARSON
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Textbook Question
Chapter 18, Problem 7R
How does the antigen in an agglutination reaction differ from that in a precipitation reaction?
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Describe the following antigen antibody reactions in serology:
Mechanism
Function in serology
Example of method
a) Precipitation
b) Agglutination
c) Neutralizing
d) Complement fixation
What is the effect of not adding the primary antibody to the ELISA reaction?
A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result be reported in terms of hemolysis at 50 %?
b) What is hydrodynamic focusing and why is it an important feature of an instrument.
Chapter 18 Solutions
EBK MICROBIOLOGY
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAECh. 18 - Prob. 4CAE
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- What is an agglutination response? How can it be avoided when blood is transfused?arrow_forwardWhat group of tests can be done to diagnose chronic myelocytic leukemia? Question 6 options: A) TRAP stain, flow cytometry, cytogenetics B) lymph node biopsy, new methlylene blue stain, sickledex C) LAP stain, flow cytometry, cytogenetics D) Sudan Black, Auramine O, Gram Stainarrow_forwardWhat is the effect of not adding the antigen to the ELISA reaction?arrow_forward
- What's Procedure of Lateral Flow Assay or Immunochromatography or Rapid Test?arrow_forwardWhat is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?arrow_forwardHow is HLA serologic testing performed? Question 28 options: a) HLA antigens move through a limited pH gradient and are immobilized, complexed with antibody, and stained. b) T lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system. c) DNA is isolated from peripheral white blood cells, fragmented, and hybridized. d) B lymphocytes are harvested and introduced to a panel of test sera specific for antigens of the HLA system.arrow_forward
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asaparrow_forwardAll of the following are used in a direct ELISA, except: A) Primary enzyme linked antibody B) Substrate C) Secondary enzyme linked antibody D) Primary antibodyarrow_forwardHow could a potential alloimmunizaton due to Anti-K be prevented? Question 45 options: a) Use of apheresed platelets b) Matching of donor and recipient red blood cell phenotype c) Use of third-generation bedside leukocyte filters d) Use of washed red blood cellsarrow_forward
- Choose all that are correct about indirect ELISA. A) An antigen can bind the substrate B) A secondary antibody can bind the antigen C) A secondary antibody can bind a primary antibody D) A primary antibody can bind an antigenarrow_forwardCompare and contrast Radial Immunodiffusion with it’s close relative, the Ouchterlony plate technique. How are they similar and how are they different?arrow_forwardName any two diagnostic kits based upon antigen.arrow_forward
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