BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 18, Problem 5U
What is a BLAST search?
a. A mechanism for aligning consensus regions during whole-genome sequencing
b. A search for similar gene sequences from other species
c. A method of screening a DNA library
d. A method for identifying ORFs
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What is Sanger sequencing? Why do we use ddNTP? How to read a DNA sequence gel? c. What is a cDNA seq (RNA seq)? d. What is the main difference between a genomic and a transcriptome study?
Check all the statements that are TRUE regarding 16S or ITS (microbiome) illumina (NGS) sequencing vs. whole genome illumina (NGS) sequencing.
A.
You don't need to trim the reads for 16S sequencing, but you do for whole genome sequencing.
B.
Whole genome sequencing files will have more reads in them because genomes are bigger than 16S amplicons.
C.
The read alignment and contig assembly steps would probably be harder for whole genome sequencing.
D.
The 16S molecules being sequenced are always DNA, while for whole genome sequencing they could be RNA or DNA going into the illumina sequencer.
E.
NGS for 16S and whole genome sequencing can both use sample indexes/barcodes to maximize efficiency.
F.
Whole genome sequencing doesn't necessarily require you to know any of the sequences/targets before hand to design specific primers for.
For the method of RNA sequencing (RNA-Seq), which of the followingis the correct order of steps?a. Isolate RNAs, synthesize cDNAs, fragment RNAs, sequencecDNAs, align cDNA sequencesb. Synthesize cDNAs, sequence cDNAs, isolate RNAs, fragmentRNAs, align cDNA sequencesc. Isolate RNAs, fragment RNAs, synthesize cDNAs, sequencecDNAs, align cDNA sequencesd. Synthesize cDNAs, isolate RNAs, fragment RNAs, sequencecDNAs, align cDNA sequences
Chapter 18 Solutions
BIOLOGY
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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- what is the whole-genome shotgun sequencing? Also briefly explain its strategy to assemble the genome sequence.arrow_forwardWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forward"Whole-Genome Sequencing Is Widely Used for Sequencing and Assembling Entire Genomes". Explain this ?arrow_forward
- Propose a method for isolating a DNA fragment that is adjacent in the genome to a previously isolated DNA fragment. Assume that you have access to a complete library of DNA fragments in a BAC vector but that the sequence of the genome under study has not yet been determined.arrow_forwardWhat is the most challenging issue facing genome sequencing? a. the inability to develop fast and accurate sequencing techniques b. the ethics of using information from genomes at the individual level c. the availability and stability of DNA d. all of the abovearrow_forwardHow the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?arrow_forward
- a. What type of nucleic acid and from what species would the scientist use to begin construction of her genomic DNA library? b. From what tissue would she isolate this nucleic acid? c. What type of reagent would the scientist use to cut the genome into appropriately sized fragments? d. What size nucleic acid fragments would one aim to prepare for the library construction so as to to avoid having to screen an overwhelming number of clones? e. Into what vector would the scientist ligate her genomic DNA fragments? f. What organism would the scientist use to propagate the clones of her genomic DNA library? g. From the information given in the problem determine what probe could be used to screen the scientist's library to find her clone of interest ?arrow_forwardQ. How can you design your RT-PCR experiment to control for gDNA contamination? A. Use forward and reverse primers that bind to the same exon. B. Run a control lane where only RT was performed and not PCR. C. Run a control lane where mRNA has been amplified using PCR. D. Use forward and reverse primers that span the junction of 2 separate exons.arrow_forwardExplain the sequencing-by synthesis (SBS) approach ?arrow_forward
- a. What are the purposes of sequence alignment? b. Define the local alignment and global alignment C. Compare local alignment and global alignment d. What is "Dot Plot" and why it is used?arrow_forwardDescribe the difference between Sanger based sequencing and Next Generation Sequencing (NGS). Why is NGS advantageous over Sanger based sequencing?arrow_forwardExplain:a. functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomalRNA (rRNA) species-specific sequences.b. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe.arrow_forward
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