BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 18, Problem 1DA
Summary Introduction
To determine: The possible combinations that allow constructing the physical map with only a few numbers of clones using the given figure.
Introduction: Physical maps give the absolute distance among genetic markers. They use genetic markers, which can show any noticeable variations between individuals. Here, the distance is measured in basepairs (bp).
Expert Solution & Answer
Explanation of Solution
The fewest number of clones that could be used to construct the physical maps is clone A and clone C. The combination of using clone A, clone B, and clone D also allows to construct physical maps. However, it is better to have all the four clones (A, B, C, and D) to verify.
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Please assign a sex type to each strain 1-8 at the bottom not to the table.
The gel image above shows 7 alleles, let's call them 1-7 in order of size, with 1 being the largest and 7 being the
smallest. What is the approximate size in Kbp of allele 4? Please type your answer as a number, please do not
add units.
ANSWER: The approximate size of allele 4 in the gel image below is Blank 1 Kbp
Photograph of UV illuminated 1 % agarose TBE gel run for 40 minutes at 120 V, showing
the result of PCR from a variable number tandem repeat region in 11 different individuals
(A-K)
2000
1650
1000
850
600
500
Key:
Lane 1:
Lanes 2-12:
A B C D E F G
H I J K
DNA ladder, see image for fragment sizes in base pairs (bp)
PCR Products from the same variable number tandem repeat (VNTR) autosomal
region of DNA from 11 different individuals (A-K)
You may insert a hand-drawn diagram of your map, but the rest of the assignment must be fully typed.
The data set attached presents the results of a testcross using female flies heterozygous for three traits and male flies, which are homozygous recessive. For simplicity, mutant alleles are shown with letters a, b, and c and wildtype alleles are indicated by a “+” symbol. For this part of the report do the following in order:
a) Determine the gene order (which gene is in the middle?)d) Construct a genetic map for the three genes, including the map distances between them. Clearly indicate the logic you followed and show all your calculations. Include the full distance calculations for the two most distanced genes (do not just add the other 2 distances).
Ensure the work is neat and clear and does not contain spelling or grammatical errors so that it is understandable.
Chapter 18 Solutions
BIOLOGY
Ch. 18.1 - Prob. 1LOCh. 18.1 - Describe the pros and cons of restriction mapping,...Ch. 18.1 - Prob. 3LOCh. 18.2 - Discriminate between dideoxy terminator sequencing...Ch. 18.2 - Prob. 2LOCh. 18.3 - Describe the findings of the Human Genome Project.Ch. 18.3 - Prob. 2LOCh. 18.3 - Prob. 3LOCh. 18.4 - Prob. 1LOCh. 18.4 - Prob. 2LO
Ch. 18.4 - Prob. 3LOCh. 18.5 - Prob. 1LOCh. 18.5 - Prob. 2LOCh. 18.5 - Prob. 3LOCh. 18.6 - Prob. 1LOCh. 18 - Prob. 1DACh. 18 - If the human genome contains approximately 3...Ch. 18 - Prob. 1IQCh. 18 - Prob. 2IQCh. 18 - Prob. 3IQCh. 18 - Prob. 4IQCh. 18 - Prob. 5IQCh. 18 - Prob. 6IQCh. 18 - A genetic map provides a. the sequence of the DNA...Ch. 18 - Prob. 2UCh. 18 - Approximately how many genes are there in the...Ch. 18 - An open reading frame (ORF) is distinguished by...Ch. 18 - What is a BLAST search? a. A mechanism for...Ch. 18 - Prob. 6UCh. 18 - Prob. 7UCh. 18 - Prob. 8UCh. 18 - Prob. 1ACh. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Prob. 4ACh. 18 - What information can be obtained from a DNA...Ch. 18 - Prob. 6ACh. 18 - Prob. 7ACh. 18 - You are in the early stages of a genome-sequencing...Ch. 18 - Genomic research can be used to determine if an...
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