Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Question
Chapter 18, Problem 4MCQ
Summary Introduction
Introduction:
ELISA (enzyme-linked immunosorbent assay) Test is used to determine and measure the antibodies in the blood. It is of two types, direct ELISA test, and indirect ELISA test. This test determines the infection for which antibodies are present in the blood.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
An ELISA was performed on the sera of three patients to detect antibody against varicella
(the chickenpox). Give the titer results shown in the answers, which patient's serum had the
most anti-varicella antibody?
There is not enough information to answer the question.
Patient 2, dilution 1:30
O Patient 1, dilution 1:2,000
O Patient 2, dilution 1:400
Figure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader
3- Discussion
1- Explain another methods by which cytotoxicity is measured ?
Describe the mechanism of an Indirect ELISA. Why is ELISA so sensitive? Why is it necessary to block unoccupied binding sites in the microtiter wells? Why is it important to have a positive control?
Chapter 18 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAE
Knowledge Booster
Similar questions
- 7 mL of a 1:50 dilution is needed to run a specific serological test. How much serum and how much diluent are needed to make this dilution? A 1:5 dilution of patient serum is necessary to run a serological test. There is 0.2 mL of serum that can be used. What amount of diluent is necessary to make this dilution using all of the serum?arrow_forwardThe secondary antibodies used in the ELISA experiment are conjugated (attached) to what enzyme? PRP HRP Rh АВОarrow_forward1. You are asked to make 150 ml of 2% agarose using a well-known buffer. How many grams will be used? a) 0.03 g b) 0.3 g c) 3 g d) 30 g 2. Place the following reactants in their proper order for the indirect ELISA test 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substratearrow_forward
- You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskarrow_forwardwhich of the following are necessary to run a valid elisa test?arrow_forwardWhat is an antigen? What is an antibody? Why does each test include a control line? What specimen would be collected for an antigen test? Why is it important to not touch the tip of the swab?What specimen would be collected for an antibody test? How many lines for a positive antigen test?How many lines for a negative antigen test? What do three lines show in an antibody test?arrow_forward
- You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).arrow_forwardDepending on the purpose, the typhoid fever can be diagnosed in the laboratory by either of the following method, i) Widal, ii. Blood culture and iii. Nucleic acid based identification method. Write a brief essay comparing these three diagnostic tests. Your answer must contain the basis of identification of each test, what does it mean by positive and negative results and which test do you think to be useful for the identification of active patient and which test is/are useful for the screening of previously infected case. Which test is prerequisite to perform the antibiotic susceptibility pattern?arrow_forwardHow does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines)arrow_forward
- ELISAs require a number of steps to be completed in order for the test to work. Why do you need to wash your samples at each step? When you added primary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen? When you added secondary antibody to the wells, what happened if your sample contained the antigen? What if it did not contain the antigen?arrow_forwardA RHO B Figure 3: Blood typing test of Unknown B. There was no agglutination observed in any of the wells. Question 4: Can Unknown A blood be safely donated to a person with Unknown B blood? Why or why not? Answer this question in the text box on Slide 5. Question 5: Can Unknown B blood be safely donated to a person with Unknown A blood? Why or why not? Answer this question in the text box on Slide 5.arrow_forwardCells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
