Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Videos
Question
Chapter 18, Problem 4MCQ
Summary Introduction
Introduction:
Brucella is a gram negative bacterium which causes Brucellosis; it is present in milk.
Brucella ELISA is technique which is used to detect the antigen, and IgG and IgM antibodies present in the Brucellosis patient serum sample. Direct ELISA test refers to the identification of the antigen. But, Indirect ELISA test refers to the identification of the antibody present in the serum sample of Brucellosis patients. Here, Brucella is the antigen.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which of the following methods can identify different strains of a microbe? a. microscopic examination b. radioimmunoassay c. serotyping d. agglutination test
Which of the following components can be used in
an ELISA?
I. Polyclonal antibodies
II. Monoclonal antibodies
III. Protein A immobilized on agarose beads
Select one:
A. II and III only
B. I only
C. Il only
D. I, II and III
E. I and II only
O
O
O
O
O
An ELISA was performed on the sera of three patients to detect antibody against varicella
(the chickenpox). Give the titer results shown in the answers, which patient's serum had the
most anti-varicella antibody?
There is not enough information to answer the question.
Patient 2, dilution 1:30
O Patient 1, dilution 1:2,000
O Patient 2, dilution 1:400
Chapter 18 Solutions
Microbiology: An Introduction
Ch. 18 - Which could cause the disease it is supposed to...Ch. 18 - Define the following terms, and give an example of...Ch. 18 - DRAW IT Label the components of the direct and...Ch. 18 - How are monoclonal antibodies produced? What is...Ch. 18 - Explain the effects of excess antigen and antibody...Ch. 18 - Prob. 6RCh. 18 - How does the antigen in an agglutination reaction...Ch. 18 - Match the following serological tests in column A...Ch. 18 - Prob. 9RCh. 18 - Prob. 10R
Ch. 18 - Prob. 1MCQCh. 18 - Prob. 2MCQCh. 18 - Prob. 3MCQCh. 18 - Prob. 4MCQCh. 18 - Prob. 5MCQCh. 18 - Prob. 6MCQCh. 18 - Prob. 7MCQCh. 18 - Test used to identify rabies virus in the brain of...Ch. 18 - Prob. 9MCQCh. 18 - Prob. 10MCQCh. 18 - What problems are associated with the use of live...Ch. 18 - Prob. 2ACh. 18 - Prob. 3ACh. 18 - Which of the following is proof of a disease...Ch. 18 - Prob. 2CAECh. 18 - Prob. 3CAECh. 18 - Prob. 4CAE
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.arrow_forwardWhat can you say about a positive latex agglutination test? Question 3 options: A It shows the patient is sick with the disease B It shows the patient has never been exposed to the disease C It shows the patient has both the antibodies and the antigen for a disease D It shows no agglutination E It shows the patient has the antibodies to a diseasearrow_forward1. What is the role of the substrate? Is it really necessary to add substrate in ELISA?Explain why, or why not.2. What is the label used in ELISA? Can we use different label? Justify your answer.3. Explain elaborately the principle involved in ELISA.4. What are the different applications of ELISA? 5. Explain the positive and negative results of ELISA.6. Why do you think it is called indirect ELISA?7. What are the different solid phases that antibody or antigen can bind to,in ELISA?8. What are the different materials needed in the experiment?9. What is theimportance of washing? When does washing is performed?10. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do?arrow_forward
- You are working in a lab studying Streptococcus pyogenes as a cause of necrotizing fasciitiis. You have an overnight culture that you want to know the starting concentration of, so do a set of six 1:10 serial dilutions (putting 1 mL from the stock into a 9 mL blank), with tube #1 being 1:10, #2 is 1:100, etc. You plate 0.1 mL from tube 5 onto a blood agar plate and the next morning count 134 colonies. How many bacteria (measured in CFU/mL) were in the overnight culture flask? A. 1.34 x 10^4 CFU/mL B. 1.34 x 10^5 CFU/mL C. 1.34 x 10^6 CFU/mL D. 1.34 x 10^7 CFU/mL E. 1.34 x 10^8 CFU/mL F. cannot tell based on the data given - you'd need to know the volume of the original culture flaskarrow_forwardYou have a suspension of fungal cells and have narrowed it down to 2 different types of fungus. You have solutions of 2 types of antibodies. 1. Antibody A recognizing and antigen on Fungus A 2. Antibody B recognizing and antigen on Fungus B The antibodies you have on hand are NOT labeled with an enzyme, dye, or fluorescence and you do NOT have sheep's blood. Which test would you do to determine this if your sample is Fungus A or Fungus B. A. precipitin B. agglutination C. complement fixation D. fluorescently labeled microscopy E. ELISAarrow_forwardRead the following study, construct the appropriate 2x2 table for evaluating a screening test, and calculate the listed statistics. Be sure to show your work. A new screening tool is being tested in European airports detect travelers who are suffering from a febrile rash illness in order to prevent passengers with measles from crossing into a different country. Three thousand (3,000) individuals were screened using this tool. All 3,000 individuals were also tested for measles using a laboratory test. There were 300 people who were positive using the screening test. There were 210 people who were positive for measles using the laboratory test. Among those with a positive laboratory test for measles, 20 tested negative with the screening tool (false negatives). 2x2 table + - Total + A B A+B - C D C+D Total A+C B+D A+B+C+D List the values for each cell: A, B, C, D, A+B, C+D, A+C, B+D, A+B+C+D Calculate the sensitivity of the test.…arrow_forward
- Match the confirmation test to the category of bacteria. (each category can be used multiple times) Categories: -Gram Positive - Gram Negative Tests: Match with category A. Citrate test is category.... B. Indole test is category... C. Raffinose test is category... D. Catalase test is category....arrow_forwardin some of the world a tuberculosis vaccine known as bacillus calmette guerin bcg is used. it is not used in the united states. every person who has received this vaccine and mounted a protective response will have a positive reaction in a tuberculin skin test. why? what does this mean for the usefulness of this skin test in those countries where this vaccine is used?arrow_forwardIn this diagram, why is anti-human IgG used in the indirect test but not in the direct test?arrow_forward
- Figure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?arrow_forwardWhich of the following is the correct pairing of an immunologic test and the subject to be identified? a. indirect ELISA test/an unknown microbial antigen b. direct fluorescence antibody test/an unknown antibody c. Western blot/an unknown microbial antigen or antibody d. agglutination test/an unknown soluble microbial toxinarrow_forwardwhat is the tuberculin test, and why is it used in clinics?arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
DNA Use In Forensic Science; Author: DeBacco University;https://www.youtube.com/watch?v=2YIG3lUP-74;License: Standard YouTube License, CC-BY
Analysing forensic evidence | The Laboratory; Author: Wellcome Collection;https://www.youtube.com/watch?v=68Y-OamcTJ8;License: Standard YouTube License, CC-BY