What is Life? A Guide to Biology with Physiology
What is Life? A Guide to Biology with Physiology
3rd Edition
ISBN: 9781464157745
Author: Jay Phelan
Publisher: W. H. Freeman
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Chapter 18, Problem 2MC
Summary Introduction

Introduction:

Plants use asexual reproduction to give rise to another, genetically identical individual. Many plant species, sexual reproduction is the predominant mode of reproduction, but nearly all plant species have also retained the ability to reproduce without any participation from another individual.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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