CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
bartleby

Concept explainers

bartleby

Videos

Question
Book Icon
Chapter 18, Problem 2AL
Summary Introduction

EMSA stands for electrophoretic mobility shift assay and it is the most common method used to study DNA-protein interactions. This method is also termed as a gel shift assay. This is because the DNA binding protein is added to the purified target DNA mixture and its mobility is slowed down using an agarose gel. The DNA-protein complex is found to be shifted to a higher molecular weight when compared to the DNA without the protein. Usually, the magnitude of the shift is related to the DNA-protein ratio. Thus, the largest shift would be seen when sufficient protein is added.

Blurred answer
Students have asked these similar questions
You generate a transgenic mouse line with a lox-stop-lox sequence upstream of a dominant-negative Notch fused to GFP. Upon crossing this mouse with another mouse line expressing ectoderm-specific Cre, what would you expect for the phenotype of neuronal differentiation in the resulting embryos?
Hair follicle formation is thought to result from a reaction-diffusion mechanism with Wnt and its antagonist Dkk1. How is Dkk1 regulated by Wnt? Describe specific cis-regulatory elements and the net effect on Dkk1 expression.
Limetown S1E4 Transcript: E n 2025SP-BIO-111-PSNT1: Natu X Natural Selection in insects X + newconnect.mheducation.com/student/todo CA NATURAL SELECTION NATURAL SELECTION IN INSECTS (HARDY-WEINBERG LAW) INTRODUCTION LABORATORY SIMULATION A Lab Data Is this the correct allele frequency? Is this the correct genotype frequency? Is this the correct phenotype frequency? Total 1000 Phenotype Frequency Typica Carbonaria Allele Frequency 9 P 635 823 968 1118 1435 Color Initial Frequency Light 0.25 Dark 0.75 Frequency Gs 0.02 Allele Initial Allele Frequency Gs Allele Frequency d 0.50 0 D 0.50 0 Genotype Frequency Moths Genotype Color Moths Released Initial Frequency Frequency G5 Number of Moths Gs NC - X
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Text book image
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Text book image
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Text book image
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Text book image
BIOLOGY:CONCEPTS+APPL.(LOOSELEAF)
Biology
ISBN:9781305967359
Author:STARR
Publisher:CENGAGE L
Text book image
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license