Chapter 18, Problem 10TYPSS

Summary Introduction

To analyze:

Colonies whose cells might have mutations from the given figure. The sequences must be analyzed for wild type or normal. Predict three types of E.coli colonies found in lactose metabolism mutants. After obtaining a mutant phenotype, the alteration to be performed for the mutant phenotype of these mutant colonies.

Introduction:

The X-gal is a colorless pigment, which can be cleaved by the β-galactosidase enzyme. The pigment is found attached to the galactose. The β-galactosidase enzyme cleaves off the X-gal pigment, which gives a blue-colored appearance.