CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 17.4, Problem 3CC
Summary Introduction

The recombinant proteins are purified by a common method called polyhistidine tagging or His-tagging. This process involves the addition of a series of histidine residues either to the C- or N- terminus of the protein. The amino acid Histidine is used for tagging because it has a high affinity for the metal ions. Proteins having multiple residues of histidine will preferentially bind to a solid phase material called resin which has exposed to cobalt or nickel atom. This allows the separation of His-tagged proteins from the other cellular constituents.

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CUSTOM PRESCOTT'S MICROBIOLOGY

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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY