PRESCOTT'S MICROBIOLOGY
PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781264073375
Author: WILLEY
Publisher: MCG
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Chapter 17.4, Problem 2CC
Summary Introduction

A gene which is been inserted into a cloning vector is referred to as a cloned gene. A cloning vector replicates independently in the host cell. The function of the gene product is often studied by cloning the gene. Cloning vectors are the vehicles that are used in carrying the foreign DNA molecules. The cloning vectors are the small piece of DNA obtained from one organism and can be stably maintained in other organisms. A foreign DNA fragment can be inserted into the cloning vector that can replicate in a host organism.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

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PRESCOTT'S MICROBIOLOGY

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