Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 17.4, Problem 10CYP
Summary Introduction
Introduction:
There are several ways in which antigen-antibody (Ag-Ab) reactions can be detected. They can be detected either by naked eyes or microscopically.
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A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating.
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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white
A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating.
For this exercise we will use seed and data from the F10 generation of a recombinant inbred population…
Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white
A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating.
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Chapter 17 Solutions
Foundations in Microbiology
Ch. 17.1 - Describe what is involved in the main categories...Ch. 17.1 - Explain several techniques in specimen collection...Ch. 17.1 - Summarize the main procedures in isolation,...Ch. 17.1 - Summarize the major techniques in identifying and...Ch. 17.1 - Describe the general principles in specimen...Ch. 17.1 - Explain why it is important to prevent microbes...Ch. 17.1 - Summarize the kinds of tests that are used to...Ch. 17.2 - Describe some direct methods of testing a...Ch. 17.2 - Summarize the aims in selection of culture...Ch. 17.2 - Prob. 6ELO
Ch. 17.2 - Describe how flowcharts and comparison tables are...Ch. 17.3 - Explain the different variations on genetic...Ch. 17.3 - Describe what is involved in direct specimen...Ch. 17.3 - Prob. 6CYPCh. 17.3 - Describe the applications of PCR in identification...Ch. 17.4 - Describe the background aims of immunologic...Ch. 17.4 - Identify how antigen-antibody reactions are...Ch. 17.4 - Prob. 11ELOCh. 17.4 - Explain the basic methods behind the Western blot...Ch. 17.4 - Prob. 13ELOCh. 17.4 - What is the basis of serology and serological...Ch. 17.4 - Differentiate between specificity and sensitivity.Ch. 17.4 - Prob. 10CYPCh. 17.4 - Prob. 11CYPCh. 17.4 - Prob. 12CYPCh. 17.4 - Prob. 13CYPCh. 17.4 - Give examples of several tests that employ...Ch. 17.4 - What is meant by complement fixation? What are...Ch. 17.4 - Prob. 16CYPCh. 17.5 - Describe the concepts behind the main types of...Ch. 17.5 - Prob. 15ELOCh. 17.6 - Prob. 16ELOCh. 17.6 - Prob. 17CYPCh. 17.6 - Prob. 18CYPCh. 17.6 - Prob. 19CYPCh. 17.6 - Prob. 20CYPCh. 17.6 - Observing figure 17.17, indicate whether each...Ch. 17.L1 - Multiple Matching. Match each of the following...Ch. 17.L1 - Prob. 2MCQCh. 17.L1 - Prob. 3MCQCh. 17.L1 - Prob. 4MCQCh. 17.L1 - A patient with a _____ titer of antibodies to an...Ch. 17.L1 - Prob. 6MCQCh. 17.L1 - Prob. 7MCQCh. 17.L1 - An example of an in vivo serological test is a....Ch. 17.L1 - Which of the following specimens must be removed...Ch. 17.L1 - Prob. 1CSRCh. 17.L1 - Prob. 2CSRCh. 17.L1 - Prob. 3CSRCh. 17.L1 - Prob. 1WCCh. 17.L1 - Prob. 2WCCh. 17.L1 - Briefly describe the principles and give an...Ch. 17.L1 - Prob. 4WCCh. 17.L1 - Prob. 5WCCh. 17.L1 - Prob. 6WCCh. 17.L2 - Prob. 1CTCh. 17.L2 - Prob. 2CTCh. 17.L2 - Why do some tests for antibody in serum (such as...Ch. 17.L2 - Prob. 4CTCh. 17.L2 - Prob. 5CTCh. 17.L2 - Prob. 6CTCh. 17.L2 - From chapter 3, fig 3.17a (reproduced on the...Ch. 17.L2 - Prob. 2VC
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