SEELEY'S ANATOMY CUSTOM LL
11th Edition
ISBN: 9781260533699
Author: VanPutte
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 17.3, Problem 15AYP
Describe and give examples of the three major ways hormone secretion is stimulated and inhibited.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
In oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be
purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the
reaction,
OTMPD is electron acceptor
O cytochrome c is the electron source
oxygen is terminal electron acceptor
OH2 produced is electron donor
Chapter 17 Solutions
SEELEY'S ANATOMY CUSTOM LL
Ch. 17.1 - Name and describe the four classes of chemical...Ch. 17.1 - Prob. 2AYPCh. 17.1 - In what ways does the nervous system differ from...Ch. 17.1 - How does an endocrine gland differ from an...Ch. 17.2 - Prob. 5AYPCh. 17.2 - Prob. 6AYPCh. 17.2 - Prob. 7AYPCh. 17.2 - What effect does a bound hormone have on the...Ch. 17.2 - What are the two chemical categories of hormones?...Ch. 17.2 - Describe how the chemical nature of a hormone...
Ch. 17.2 - Prob. 11AYPCh. 17.2 - Why do organs regulated by protein hormones have...Ch. 17.2 - Prob. 13AYPCh. 17.2 - Describe chronic, acute, and episodic patterns of...Ch. 17.3 - Describe and give examples of the three major ways...Ch. 17.3 - Prob. 16AYPCh. 17.4 - What characteristics ofa hormone receptor make...Ch. 17.4 - What is down-regulation, and what may cause it to...Ch. 17.4 - Prob. 19AYPCh. 17.4 - What are the two classes of hormone receptors? How...Ch. 17.4 - Prob. 21AYPCh. 17.4 - Prob. 22AYPCh. 17.4 - Prob. 23AYPCh. 17.4 - What two ways can a membrane-bound receptor use to...Ch. 17.4 - Explain how the hormone-receptor complex can alter...Ch. 17.4 - List four intracellular mediators affected by G...Ch. 17.4 - Prob. 27AYPCh. 17.4 - Prob. 28AYPCh. 17.4 - Prob. 29AYPCh. 17.4 - Prob. 30AYPCh. 17 - Prob. 1RACCh. 17 - Prob. 2RACCh. 17 - Which of these can regulate the secretion of a...Ch. 17 - Prob. 4RACCh. 17 - Prob. 5RACCh. 17 - Concerning the half-lifeof hormones, a....Ch. 17 - Prob. 7RACCh. 17 - Prob. 8RACCh. 17 - Prob. 9RACCh. 17 - Prob. 10RACCh. 17 - Prob. 11RACCh. 17 - Which of these can limit a cell's response to a...Ch. 17 - Prob. 13RACCh. 17 - Prob. 14RACCh. 17 - When a hormone binds to a nuclear receptor a. DNA...Ch. 17 - Prob. 16RACCh. 17 - Prob. 1CTCh. 17 - Prob. 2CTCh. 17 - Prob. 3CTCh. 17 - Prob. 4CTCh. 17 - Prob. 5CTCh. 17 - Prob. 6CTCh. 17 - Thyroid hormones are important in regulating the...Ch. 17 - Prob. 8CTCh. 17 - Prob. 9CT
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