Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Textbook Question
Chapter 17.2, Problem 1CC
Compare the effect on the host cell of a ly1ic (virulent) phage and a lysogenic (temperate) phage
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Describe the reason behind the occurrence of phage growth and release by the mating with the non-lysogenic recipient, but not with the infection by a lysogenic recipient.
During the lysogenic cycle of lambda (1) phage
the phage genome is integrated in the host chromosome where it is passed on to daughter cells through binary fission.
the phage DNA is copied and accumulates in the cytoplasm. Then a trigger causes capsid proteins to be made and phages are assembled.
the product of one prophage gene is made and it activates most of the other prophage genes.
the viral genes immediately turn the host cell into a lambda-producing factory. Then the host lyses.
0000
phage can be "induced" as a result of DNA damage. Consequences of
induction include.
Temperate phage, e.g lambda phage; lytic cycle and transduction
Lytic T4 phage, assembly, conjugation
O Lytic phage, e.g. T4 phage; lysogenic cycle, prophage formation
Temperate phage, e.g. lambada phage, prophage formation, transformation
O T4 phage, lytic cycle, specialized trasnduction
Chapter 17 Solutions
Campbell Biology in Focus
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- Compare and contrast the lysogenic and lytic cycles of phage λ.arrow_forwardWhat advantages might a phage gain by being capable of lysogeny?arrow_forwardWhat is the difference between the lytic and lysogenic cycle of bacteriophages? In the lysogenic cycle, the phage DNA integrates itself into the host genome after penetration, and continues to biosynthesis when conditions change The host cell is not killed in the lysogenic cycle The lytic cycle occurs in the nucleus, while the lysogenic cycle occurs in the cytoplasm In the lysogenic cycle, penetration is achieved through phagocytosisarrow_forward
- Lambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).arrow_forwardDescribe in detail the kinetics of phage infection followed by host lysis in bacteria. with examplesarrow_forwardHost DNA is digested into small pieces, which are occasionally assembled with phage proteins, creating a phage with bacterial chromosomal DNA. If the breakage of the chromosomal DNA is not random (i.e., it is more likely tobreak at certain spots as opposed to other spots), how might suchnonrandom breakage affect cotransduction frequency?arrow_forward
- Indicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1. The host cell is lysed. (Click to select) v 2. The genetic material of the phage is copied. (Click to select) v 3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v 4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v 5. The integrase gene is turned on.: (Click to select) v (Click to select) lytic lysogenic botharrow_forwardClassify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.arrow_forwardA researcher is studying the r// locus of phage T4. Three //-- strains are obtained: A, B, and C. E. coli strain K(A) is coinfected with two rl strains simultaneously and the results are recorded. Infection with A and B phage = lysis occurs Infection with A and C phage = lysis occurs Infection with B and C phage = no lysis occurs What can be inferred from this complementation test? Multiple Choice Strains A, B, and C have mutations in three different genes. Strains A and B have mutations in the same gene, and strain C has a mutation in a different gene. Strains A and C have mutations in the same gene, and strain B has a mutation in a different gene. Strains A, B, and C have mutations in the same gene. Strains B and C have mutations in the same gene, and strain A has a mutation in a different gene.arrow_forward
- When a temperate phage infects a bacteria cell it O immediately undergoes the temperate lifecycle. O immediately integrates its genome into the host cell genome; when conditions are harsh, the phage excises out of the host chromosome and undergoes the lytic lifecycle. O immediately undergoes the lytic lifecycle. O can either immediately undergo the lytic lifecycle or immediately undergo the lysogenic lifecycle, and it usually undergoes the lytic lifecycle.arrow_forwardConsider the phase variation of flagella in Salmonella. Describe the phenotype of a cell in which hin is deleted. Will it be motile? Will it undergo phase variation? Which flagellin(s) will be used, if any? Name two bacterial defenses against bacteriophages and explain how they work How might a phage evolve resistance to the CRISPR host defense?arrow_forwardDescribe how penetration occurs in T-4 phages.arrow_forward
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