Whether the gene GAL4 or GAL80 genes can be considered as potential candidates for isolation of mutations that allow the constitutive expression of the target genes along with the characteristic of protein that disrupts the mutation. Introduction: Yeast genes have cis-acting elements upstream of their promoters and are similar to enhancers, which are called upstream activating sequences (UASs). UAS G regulates the several target genes involved in the galactose utilization.

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Alleles at the P locus control seed color. Plants which are pp have white seeds, white flowers and no pigment in vegetative parts. Plants which are P_ have black seeds, purple flowers and may have varying degrees of pigment on stems and leaves. Seed color can be assessed, visually, based on if the seed is white or not white A gene for mold resistance has been reported and we want to determine its inheritance and whether it is linked to P. For the purposes of this exercise, we will assume that resistance is controlled by a single locus M, and M_ plants are resistant and mm plants are susceptible. Resistance can be measured, under greenhouse conditions, 2 weeks after planting, by injecting each seedling with a spore suspension. After two weeks, the seedlings can be rated as resistant or susceptible, based on whether or not tissue is actively sporulating. For this exercise we will use seed and data from the F10 generation of a recombinant inbred population produced using single seed…

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Question

Chapter 17, Problem 8P

Summary Introduction

To determine:

Whether the gene GAL4 or GAL80 genes can be considered as potential candidates for isolation of mutations that allow the constitutive expression of the target genes along with the characteristic of protein that disrupts the mutation.

Introduction:

Yeast genes have cis-acting elements upstream of their promoters and are similar to enhancers, which are called upstream activating sequences (UASs). UAS_G regulates the several target genes involved in the galactose utilization.

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