Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781260081848
Author: Robert Brooker
Publisher: Mcgraw-hill Higher Education (us)
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Chapter 17, Problem 7TY
Summary Introduction

Introduction:

Bacteria are the most abundant and oldest microorganisms. They have the ability to sustain in diverse environmental conditions. They have a very simple cell structure but quite complex nature and behavior.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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Bacterial Structure and Functions; Author: Osmosis;https://www.youtube.com/watch?v=b15Hy3jCPDs;License: Standard youtube license