The fragments obtained from the cleavage of given peptide chain by trypsin, cyanogen bromide and chymotrypsin should be given. Concept introduction: Amino acids are linked each other by peptide bonds is called protein (one or more peptide bonds). Peptide bonds are amide bonds, in which carbonyl group of one amino acid bonded to amino group of other amino acid. The chain of the protein is drawn in such a way that left end is occupied by free amino group (say N-terminal) and right side of the chain is occupied by carbonyl group (say C-terminal). Endopeptidases cleave peptide bonds that are not at the end. Trypsin is an endopeptidase, which cleave the C-side peptide bond of positively charged side chains such as arginine and lysine. Chymotrypsin is an endopeptidase, which cleave the C-side peptide bond of amino acids that contain aromatic six-membered rings such as Phe, Tyr and Trp. Cyanogen bromide cleaves the C-terminal peptide of Methionine in a peptide chain.

Question

Want to see the full answer?

Answer 1

Question

Chapter 17, Problem 55P

(a)

Interpretation Introduction

Interpretation:

The fragments obtained from the cleavage of given peptide chain by trypsin, cyanogen bromide and chymotrypsin should be given.

Concept introduction:

Amino acids are linked each other by peptide bonds is called protein (one or more peptide bonds). Peptide bonds are amide bonds, in which carbonyl group of one amino acid bonded to amino group of other amino acid. The chain of the protein is drawn in such a way that left end is occupied by free amino group (say N-terminal) and right side of the chain is occupied by carbonyl group (say C-terminal).

Endopeptidases cleave peptide bonds that are not at the end.

Trypsin is an endopeptidase, which cleave the C-side peptide bond of positively charged side chains such as arginine and lysine.

Chymotrypsin is an endopeptidase, which cleave the C-side peptide bond of amino acids that contain aromatic six-membered rings such as Phe, Tyr and Trp.

Cyanogen bromide cleaves the C-terminal peptide of Methionine in a peptide chain.

(b)

Interpretation Introduction

Interpretation:

The numbers of primary structure if the amino acids contained in each fragment are known has to be determined.

Concept introduction:

The number of primary structures in a polypeptide is determined by checking the number of disulfide bridges connected with primary structures.

Disulfide bridges are formed between thiol groups of cysteine.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

" is The structure of the bicarbonate (hydrogen carbonate) ion, HCO3-, HCO3 best described as a hybrid of several contributing resonance forms, two of which are shown here. HO :0: :Ö: HO + Bicarbonate is crucial for the control of body pH (for example, blood pH: 7.4). A more self-indulgent use is in baking soda, where it serves as a source of CO2 CO₂ 2 gas, which gives bread and pastry their fluffy constituency. (i) Draw at least one additional resonance form. = (ii) Using curved "electron-pushing" arrows, show how these Lewis structures may be interconverted by movement of electron pairs. (iii) Determine which form or forms will be the major contributor(s) to the real structure of bicarbonate, explaining your answer on the basis of the criteria in Section 1-5.

Which of these is the best use of a volumetric flask? measuring how much liquid it contains delivering a precise amount of liquid to another container holding solutions making solutions of precise concentration

You're competing on a Great British television game show, and you need to bake a cake. The quantity for each ingredient is given in grams, but you haven't been given a kitchen scale. Which of these properties would correlate with the mass of a baking ingredient like eggs or milk? Check all that apply. depth of color viscosity volume density