
Concept explainers
A.
To explain: The changes at the end of shrinking phase microtubule in order to stop its shrinking and to start growing again.
Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.
B.
To explain: How the changes in the tubulin concentration affect the shrinking phase microtubule.
Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.
C.
To explain: The results when the solution contained only GDP and not GTP.
Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.
D.
To explain: The results if the solution contains an analog GTP that cannot be hydrolyzed.
Introduction: Dynamic instability is characterized by interchange between phases of relatively slow growing and rapid shortening at the ends of individual microtubules. It is a result of the hydrolysis of guanosine triphosphate (GTP)-bound to β-tubulin shortly after assembly. The microtubules grow rapidly when GTP caps are present in its end, whereas the microtubules that are associated with guanosine diphosphate (GDP)-tubulin heterodimers at their ends are rapidly depolymerized.

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Chapter 17 Solutions
Essential Cell Biology 5e
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