EBK CONCEPTS OF GENETICS
EBK CONCEPTS OF GENETICS
12th Edition
ISBN: 9780134818979
Author: Killian
Publisher: YUZU
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Chapter 17, Problem 22ESP

(a)

Summary Introduction

To determine: The way by which deletion within the GAL4 gene would affect transcription of the yeast GAL1 gene in the presence of galactose.

Introduction: Transcription is the process of formation of RNA (ribonucleic acid) from the complementary DNA (deoxyribonucleic acid). GAL genes are required for the proper growth of yeast in galactose medium. The regulation of GAL genes is regulated by the products of GAL4, GAL8, and GAL3 genes. Mutation in these regions may disrupt the process of transcription in yeast.

(b)

Summary Introduction

To determine: The way by which deletion within the entire GAL3 gene would affect transcription of the yeast GAL1 gene in the presence of galactose.

Introduction: As mentioned in the concept introduction part a.

(c)

Summary Introduction

To determine: The way by which deletion of a mutation within the GAL80 gene that blocks the ability of Gal80 protein to interact with Gal3p would affect transcription of the yeast GAL1 gene in the presence of galactose.

Introduction: As mentioned in the concept introduction part a.

(d)

Summary Introduction

To determine: The way by which deletion of one of the four UASG elements upstream from the GAL1 gene would affect transcription of the yeast GAL1 gene in the presence of galactose.

Introduction: As mentioned in the concept introduction part a.

(e)

Summary Introduction

To determine: The way by which a point mutation in the GAL1 core promoter that alters the sequence of the TATA box would affect transcription of the yeast GAL1 gene in the presence of galactose.

Introduction: As mentioned in the concept introduction part a.

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Question #3: In the KeyGene paper, the authors state that it would be useful if pollen from an apomict would transmit apomixis-inducing genes to the female in the cross (assuming the pollen is viable). Assuming there was just one gene conferring gametophytic obligate apomixis, and that the two parents are inbreds, what would be the consequences of such a cross if: a) The apomixis was a dominant trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the parental cross. b) The apomixis was a recessive trait? Indicate the genotypes and phenotypes (apomict or non- apomict) of the parents, F1 and F2 generations. Remember to include the expected genotypic and phenotypic ratios (or percentages) in the F1 and F2 generations, and to position the female first (left side) in the…

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EBK CONCEPTS OF GENETICS

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