The site where the largest amount of biologically active energy is available in triacylglycerols. in . Introduction: The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

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Answer 1

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Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

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Draw a diagram to demonstrate 3' to 5' exonuclease activity?

1. Write the piece of mRNA that would code for the amino acid sequence NH3 - met - tyr - cys- his- CO2. Label the 5' and 3' ends. (diagram attached) 2.

You were tasked with purifying an untagged transcription factor (molecular weight 65,000Da, isoelectric point unknown) from a contaminant Protein A (molecular weight 50,000 Da, isoelectric point 5.0). You were also instructed to use the protein for crystallization studies after purification.i) In the initial purification step, which type of chromatography should you attempt? Explain your choice and specify the requirements of the buffer solution you should use. ii) Analyzing the proteins recovered from step i) using SDS-PAGE, you still observed a faint band of Protein A in addition to the transcription factor band. Given the limited time available for further purification, you must choose ONE appropriate chromatography method to maximize the chances of separating the two proteins as well as your crystallization studies. Provide a detailed explanation of your selection and the techniques/strategies you would employ to achieve this.

Answer 2

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Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

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Answer 3

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Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

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Answer 4

Question

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Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

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Answer 5

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Answer 6

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Answer 7

Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

Answer 8

Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Answer 9

Chapter 17, Problem 1P

Summary Introduction

To determine: The site where the largest amount of biologically active energy is available in triacylglycerols. in .

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglycerides or triacylglycerols are made up of three molecules of fatty acids and one glycerol molecule.

Answer 10

Expert Solution

Explanation of Solution

The process of oxidation of fatty acids results in electron release from fatty acid which moves through respiratory chain to form molecules of ATP

The released fatty acid is then oxidized by the process of beta oxidation to form acetyl co enzyme A which enters the citric acid cycle and releases energy. Biologically available energy present in fatty acid chains is about 95% whereas it is only 5% in glycerol molecules. Thus, fatty acid is the site where largest amount of biologically active available energy is stored.

Answer 11

Expert Solution

Answer 12

Expert Solution

Answer 13

Expert Solution

Answer 14

Conclusion

Fatty acid in triacylglycerols is the site where most of the biologically available energy is stored.

Answer 15

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.

Answer 16

Summary Introduction

To explain: The way in which the knowledge of chemical structure of triacylglycerols helps in determining the site of largest amount of biological energy in triacylglycerols.

Introduction:

The most common form of lipid present in diet is a fat called triglycerides. Triglyceride or triacylglycerols is made up of three molecules of fatty acid and one glycerol molecule.

Answer 17

Expert Solution

Explanation of Solution

The structural characteristics of fatty acid make them the site for large amount of energy. Long alkyl chains of fatty acids are greatly reduced that have high oxidation energy. The carbon molecules in fatty acid are greatly reduced as compared to glycerol.