EBK GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260041255
Author: HARTWELL
Publisher: MCGRAW HILL BOOK COMPANY
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Question
Chapter 17, Problem 17P
Summary Introduction
To determine:
The way in which a strain of yeast is used to identify the prey proteins along with the ways in which this protocol is relevant to the goal of finding the proteins.
Introduction:
The yeast two-hybrid system method has three components. First: the yeast that contains a reporter gene construct in which UASG drives the expression of an E.Coli. Second: the yeast expresses the fusion protein in which the DNA binding domain of GAL4 is fused to the protein of interest. Third: a cDNA library made in plasmids where each cDNA is fused in frame to the activation domain of GAL4.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The metabolic pathway below is used for the production of the purine nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) in eukaryotic cells. Assume each arrow represents a reaction catalyzed by a different enzyme. Using the principles of feedback inhibition, propose a regulatory scheme for this pathway that ensures an adequate supply of both AMP and GMP, and prevents the buildup of Intermediates A through G when supplies of both AMP and GMP are adequate.
QUESTION 27
Label the structures marked A, B, C and explain the role of structure A.
W
plasma membrane
For the toolbar, press ALT+F10 (PC) or ALT+FN+F10 (Mac).
BIUS
☐
Paragraph
Π " ΩΘΗ
Β
Open Sans, a...
10pt
EE
examples of synamptomorphy
Chapter 17 Solutions
EBK GENETICS: FROM GENES TO GENOMES
Ch. 17 - For each of the terms in the left column, choose...Ch. 17 - For each of the following types of gene...Ch. 17 - List five events other than transcription...Ch. 17 - Which eukaryotic RNA polymerase RNA pol I, pol II,...Ch. 17 - As shown in the following diagram, a single...Ch. 17 - You have synthesized an enhancerless GFP reporter...Ch. 17 - Prob. 7PCh. 17 - Prob. 8PCh. 17 - A single UAS regulates the expression of three...Ch. 17 - MyoD is a transcriptional activator that turns on...
Ch. 17 - a. Assume that two transcription factors are...Ch. 17 - Prob. 12PCh. 17 - In Problem 12, you identified a genomic region...Ch. 17 - Prob. 14PCh. 17 - Prob. 15PCh. 17 - Genes in both prokaryotes and eukaryotes are...Ch. 17 - Prob. 17PCh. 17 - Lysine 4 of histone H3 H3K4 is methylated in the...Ch. 17 - J.T. Lis and collaborators have developed an...Ch. 17 - Hydatiform moles are growths of undifferentiated...Ch. 17 - Prader-Willi syndrome is caused by a mutation in...Ch. 17 - The human IGF2 gene is autosomal and maternally...Ch. 17 - Follow the expression of a paternally imprinted...Ch. 17 - Reciprocal crosses were performed using two inbred...Ch. 17 - Interestingly, imprinting can be tissue-specific....Ch. 17 - Prob. 26PCh. 17 - A method for detecting methylated CpGs involves...Ch. 17 - Honeybees Apis mellifera provide a striking...Ch. 17 - Consider the experiment in Fig. 17.24, where the...Ch. 17 - A protein or RNA that regulates gene expression in...Ch. 17 - a. How can a single eukaryotic gene give rise to...Ch. 17 - A hunchback gene, a gene necessary for proper...Ch. 17 - You know that the mRNA and protein produced by a...Ch. 17 - You are studying a transgenic mouse strain that...Ch. 17 - Prob. 35PCh. 17 - Scientists have exploited the siRNA pathway to...Ch. 17 - Persimmons Diospyros lotus are dioecious plants,...Ch. 17 - Drosophila females homozygous for loss-of-function...Ch. 17 - The text has discussed the RNA-Seq technique,...Ch. 17 - Researchers know that Fru-M controls male sexual...Ch. 17 - The Drosophila gene Sex lethal Sxl is deserving of...Ch. 17 - Figure 17.29 shows that the Sxl protein binds to...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- examples of synamtomorphy.arrow_forwardE. Bar Graph Use the same technique to upload the completed image. We will use a different type of graph to derive additional information from the CO2 data (Fig A1.6.2) 1. Calculate the average rate of increase in COz concentration per year for the time intervals 1959-1969, 1969- 1979, etc. and write the results in the spaces provided. The value for 1959-1969 is provided for you as an example. 2. Plot the results as a bar graph. The 1959-1969 is plotted for you. 3. Choose the graph that looks the most like yours A) E BAR GRAPH We will use a different type of graph to derive additional information from the CU, data (rig. nive). Average Yearly Rate of Observatory, Hawall interval Rate of increase per year 1959-1969 0.9 1969-1979 1979-1989 1989-1999 1999-2009 Figure A1.6.2 1999-2009 *- mrame -11- -n4 P2 جية 1989-1999 1979-1989 1969-1979 1959-1969 This bar drawn for you as an example 1.0 CO, Average Increase/Year (ppmv) B) E BAR GRAPH We will use a different type of graph to derive…arrow_forwardUse the relationships you just described to compute the values needed to fill in the blanks in the table in Fig A1.4.1 depth (a) 1.0 cml 0.7 cml cm| base dimensions (b, c)| 1.0 cm| 1.0 cm| 1.0 cm 1.0 cm| 1.0 cm| 1.0 cm volume (V) 1.0_cm' cm'| cm'| density (p) 1.0 g/cm'| 1.0 g/cm 1.0 g/cm' mass (m)| 0.3 g Column 1: depth at 1.0 cm volume mass Column 2: depth at 0.7 cm volume mass Column 3: unknown depth depth volumearrow_forward
- San Andreas Transform Boundary Plate Motion The geologic map below of southern California shows the position of the famous San Andreas Fault, a transform plate boundary between the North American Plate (east side) and the Pacific Plate (west side). The relative motion between the plates is indicated by the half arrows along the transform plate boundary (i.e., the Pacific Plate is moving to the northwest relative to the North American Plate). Note the two bodies of Oligocene volcanic rocks (labeled Ov) on the map in the previous page located along either side of the San Andreas Fault. These rocks are about 23.5 million years old and were once one body of rock. They have been separated by displacement along the fault. 21. Based on the offset of these volcanic rocks, what is the average annual rate of relative plate motion in cm/yr? SAF lab 2.jpg Group of answer choices 0.67 cm/yr 2 cm/yr 6.7 cm/yr 1.5 cm/yr CALIFORNIA Berkeley San Francisco K Os Q San Andreas Fault Ov…arrow_forwardThese are NOT part of any graded assignment. Are there other examples of synapomorphy. What is it called when the traits retained are similar to ancestors?arrow_forwardPlease hand draw everying. Thank you! Draw a gram positive bacterial cell below. Your cell should have the following parts, labeled: A coccus shape A capsule The gram positive cell wall should have the peptidoglycan labeled, as well as its component parts (NAM, NAG, and teichoic acid) A cell membrane Fimbriae A nucleoid Ribosomes Inclusionsarrow_forward
- Draw a gram negative bacterial cell below. Your cell should have the following parts, labeled: A bacillus shape Fimbriae Amphitrichous flagella 2 membranes (outer and inner) The outer membrane should have lipopolysaccharide (LPS) with lipid A and O antigens Periplasmic space The thin peptidoglycan cell wall between the 2 membranes A nucleoid Ribosomes Inclusionsarrow_forwardBacterial species Cell wall type Example: S. mitis Gram positive S. epidermidis H. pylori M. bovis S. marcescens Shape and arrangement Coccus, streptococcus Drawing 0000000arrow_forwardDraw a gram positive bacterial cell below. Your cell should have the following parts, labeled: A coccus shape A capsule The gram positive cell wall should have the peptidoglycan labeled, as well as its component parts (NAM, NAG, and teichoic acid) A cell membrane Fimbriae A nucleoid Ribosomes Inclusionsarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Biology: The Dynamic Science (MindTap Course List)BiologyISBN:9781305389892Author:Peter J. Russell, Paul E. Hertz, Beverly McMillanPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage Learning
Biology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning
Human Biology (MindTap Course List)BiologyISBN:9781305112100Author:Cecie Starr, Beverly McMillanPublisher:Cengage Learning
Biology: The Dynamic Science (MindTap Course List)
Biology
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Biology (MindTap Course List)
Biology
ISBN:9781337392938
Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Biochemistry
Biochemistry
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Cengage Learning
Human Biology (MindTap Course List)
Biology
ISBN:9781305112100
Author:Cecie Starr, Beverly McMillan
Publisher:Cengage Learning
Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY