SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
9th Edition
ISBN: 9781319398583
Author: BERG
Publisher: MAC HIGHER
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Chapter 17, Problem 12P
Interpretation Introduction

Interpretation:

The fate of the radioactive label carbon atoms in the given compounds if they are added to a cell extract which contains the enzyme and cofactors of the glycolytic pathway, the citric acid cycle and pyruvate dehydrogenase complex is to be stated.

Concept introduction:

The cycle which represents the set of chemical reactions that are utilized by all the aerobic or living organisms which helps them in releasing the stored amount of energy by the oxidation process of acetyl-CoA is known as citric acid cycle. This acetyl-CoA is obtained from the carbohydrates, protein and fats in the form of ATP.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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