a.
To determine:
The basis for tRNA synthetase genes in B. subtilis regulated by T-box RNA leaders.
Introduction:
The promoter of the lac operon functions as the binding site for RNA polymerase. The RNA polymerase enzyme performs the function of transcription. The operator acts as a negative regulatory site and forms complex with lac repressor protein. The operator passes over the promoter, and when the repressor is bound then the RNA polymerase is not able to bind with the promoter and thus, starts transcription.
b.
To determine:
The alteration in the tyrS T-box leader so that it can respond to the uncharged tRNAPhe instead of tRNATyr.
Introduction:
DNA is the genetic material of all organisms except some viruses.
c.
To determine:
A change in the interaction of the 5’ UAC 3’ codon when it was changed to 5’ CUA 3’.
Introduction:
The codons are a set of three nucleotides that encode for a specific amino acid. There are a total of 64 codons that can account for 20 amino acids. The amino acid tyrosine is encoded by only one amino acid.
d.
To determine:
The gene mutation that can make the mutant T-box RNA leader functional again.
Introduction:
The process of DNA replication is the process by which the genetic material of the organism copies itself to be distributed among the daughter cells during cell division. The DNA replication takes place before the cell division and prepares the cell for mitosis and meiosis. This process is followed by transcription and translation.
e.
To determine:
The effect on beta-galactosidase when the levels of tyrosine are high and low.
Introduction:
Proteins are the long chains of amino acids that are present in a specific sequence in the cell. These protein are synthesized by mRNA inside the nucleus and then transported into the cytoplasm where they undergo post-translational modifications.
f.
To determine:
The effect on beta-galactosidase when the levels of tyrosine are high and low when the mutation takes place in the tRNA gene.
Introduction:
The process of DNA replication is the process by which the genetic material of the organism copies itself to be distributed among the daughter cells during cell division. The DNA replication results in the production of multiple copies of DNA that is distributed among daughter cells.
g.
To determine:
The search criteria for T-box regulators.
Introduction:
The process of DNA replication is the process by which the genetic material of the organism copies itself to be distributed among the daughter cells during cell division. The DNA replication takes place before the cell division and prepares the cell for mitosis and meiosis. This process is followed by transcription and translation.

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Chapter 16 Solutions
GENETICS:FROM GENES TO GENOMES(LL)-PKG
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