Lehninger Principles Of Biochemistry 7e & Study Guide And Solutions Manual For Lehninger Principles Of Biochemistry 7e
Lehninger Principles Of Biochemistry 7e & Study Guide And Solutions Manual For Lehninger Principles Of Biochemistry 7e
7th Edition
ISBN: 9781319125776
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of 14C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of 14C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of 14C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of 14C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of 14C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of 14C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of 14C in Oxaloacetate. 

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

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Macmillan Learning Cholesterol synthesis begins with the formation of mevalonate from acetyl CoA. This process activates mevalonate and converts it to isopentenyl pyrophosphate. Identify the atoms in mevalonate and isopentenyl pyrophosphate that will be labeled from acetyl CoA labeled with 14C in the carbonyl carbon. Place 14C atoms and C atoms to denote which carbon atoms are labeled and which are not labeled. H₂C COA 14C-labeled acetyl-CoA HHH [c] H H OH 014C - OH H HH H Mevalonate CH3 H H 14C H Η H H Incorrect Answer of o -P-O-P-0- Isopentenyl pyrophosphate с Answer Bank 14C
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