Whether added glucose in isolated mitochondria suspension is metabolized into H 2 O and CO 2. Introduction : Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

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Chapter 16, Problem 16P

(a)

Summary Introduction

To determine: Whether added glucose in isolated mitochondria suspension is metabolized into H₂O and CO_2.

Introduction:

Isotope labeling is a technique which is used to locate the path of an isotope in a metabolic pathway or reaction. In this technique, the reactant substrate is labeled. The labeling refers to the replacement of specific atom by their isotopes.

(b)

Summary Introduction

To determine: The location of ¹⁴C in the oxaloacetate after one turn of citric acid cycle when a brief pulse of [3-¹⁴C] pyruvate is added into mitochondria. Also determine the number of turns of cycle required to release all the [3-¹⁴C] pyruvate as CO₂.

Introduction:

The series of metabolic reactions in which the energy stored in the compounds is released for the production of ATP (adenosine triphosphate), is called “Krebs’s cycle or citric acid cycle”.

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