Location of 14 C in fructose-1,6-bisphosphate. Introduction : In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

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Answer 1

Question

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

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Answer 3

Question

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

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See solution

Answer 4

Question

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

Answer 6

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

Answer 7

Chapter 16, Problem 18P

(a)

Summary Introduction

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

Answer 8

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

Answer 9

(b)

Summary Introduction

To determine: Location of ¹⁴C in glyceraldehyde-3-phosphate.

Introduction:

Glycolysis cycle starts from glucose which is a six carbon membered structure. Fructose-1,6-bisphosphate gets converted into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. These structures are 3-carbon substances.

Answer 10

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

Answer 11

(c)

Summary Introduction

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

Answer 12

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

Answer 13

(d)

Summary Introduction

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

Answer 14

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

Answer 15

(e)

Summary Introduction

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

Answer 16

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

Answer 17

(f)

Summary Introduction

To determine: Location of ¹⁴C in α-Ketoglutarate.

Introduction:

α-Ketoglutarate is obtained as the intermediate of the citric acid cycle. Isocitrate is obtained by the activity of aconitase on citrate. The obtained isocitrate is converted into α-Ketoglutarate in the presence of isocitrate dehydrogenase.

Answer 18

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

Answer 19

(g)

Summary Introduction

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

Answer 20

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Answer 21

Expert Solution & Answer

Answer 22

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Answer 23

Ch. 16 - Prob. 1P Ch. 16 - Prob. 2P Ch. 16 - Prob. 3P Ch. 16 - Prob. 4P Ch. 16 - Prob. 5P Ch. 16 - Prob. 6P Ch. 16 - Prob. 7P Ch. 16 - Prob. 8P Ch. 16 - Prob. 9P Ch. 16 - Prob. 10P

Location of 14 C in fructose-1,6-bisphosphate. Introduction : In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

To determine: Location of 14C in fructose-1,6-bisphosphate. Introduction: In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

To determine: Location of 14C in phosphoenolpyruvate (PEP). Introduction: Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

To determine: Location of 14C in acetyl CoA. Introduction: Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

To determine: Location of 14C in citrate. Introduction: Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

To determine: Location of 14C in Oxaloacetate. Introduction: Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.

Want to see the full answer?

Chapter 16 Solutions

To determine: Location of ¹⁴C in fructose-1,6-bisphosphate.

Introduction:

In the glycolysis process, glucose is catabolized to release energy. Glucose serves as the prime carbon source for the cells. Citric acid cycle is also involved with the release of energy.

To determine: Location of ¹⁴C in phosphoenolpyruvate (PEP).

Introduction:

Glyceraldehyde 3- phosphate is obtained at the fourth step of glycolysis. Glyceraldehyde 3- phosphate further undergoes conversion into 1,3- bisphospho glycerate and eventually phosphoenol pyruvate (PEP) is obtained.

To determine: Location of ¹⁴C in acetyl CoA.

Introduction:

Acetyl CoA is the compound which enters the citric acid cycle along with oxaloacetate. The first product of citric acid cycle is citrate. Citric acid cycle occurs in anaerobic conditions.

To determine: Location of ¹⁴C in citrate.

Introduction:

Citrate is the first product of citric acid cycle. Oxaloacetate combines with acetyl CoA in the presence of citrate synthase and produces citrate.

To determine: Location of ¹⁴C in Oxaloacetate.

Introduction:

Two molecules of pyruvate give rise to two molecules of oxaloacetate. Pyruvate carboxylase works on pyruvate to produce oxaloacetate. Pyruvate combines with bicarbonate to produce oxaloacetate.