Determine the approach by which two-hybrid method facilitate the discovery of protein- protein interactions. Explain the reason for the false-positive results (where the proteins encoded by two clones interact in the two-hybrid system but do not interact in the organism in which they naturally exist) obtained sometimes. Also the reason for the occurrence of false – negative (where the two proteins interact in vivo but fail to interact in the two-hybrid system) results. Introduction: There are many high-throughput technologies developed for proteomic analyses, including techniques to study protein expression, modification, and protein-protein interactions. The study of protein- protein interactions show whether and how different proteins interact, and provide information on the functioning of biological systems by identifying sets of proteins that form complexes. Two hybrid methods are successfully applied to many model systems, providing information on their protein interaction networks.

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Chapter 16, Problem 13P

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Determine the approach by which two-hybrid method facilitate the discovery of protein- protein interactions. Explain the reason for the false-positive results (where the proteins encoded by two clones interact in the two-hybrid system but do not interact in the organism in which they naturally exist) obtained sometimes. Also the reason for the occurrence of false – negative (where the two proteins interact in vivo but fail to interact in the two-hybrid system) results.

Introduction:

There are many high-throughput technologies developed for proteomic analyses, including techniques to study protein expression, modification, and protein-protein interactions. The study of protein- protein interactions show whether and how different proteins interact, and provide information on the functioning of biological systems by identifying sets of proteins that form complexes. Two hybrid methods are successfully applied to many model systems, providing information on their protein interaction networks.

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