EBK PRINCIPLES OF HUMAN PHYSIOLOGY
6th Edition
ISBN: 8220101472250
Author: STANFIELD
Publisher: PEARSON
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Chapter 15.3, Problem 15.1.4QC
Summary Introduction
To write:
The roles of the various organs involved in the formation of RBC which also help in maintaining the normal RBC level in blood.
Introduction:
Erythrocytes are one of the major components of blood. The main function of erythrocytes is to carry oxygen and carbon dioxide to and from the cells and tissues. The erythrocytes are also known as Red Blood Cells (RBCs) and they have a protein known as hemoglobin which binds the oxygen molecules and helps in its transfer to different cells and tissues.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 15 Solutions
EBK PRINCIPLES OF HUMAN PHYSIOLOGY
Ch. 15.3 - Prob. 15.1.1QCCh. 15.3 - Prob. 15.1.2QCCh. 15.3 - Prob. 15.1.3QCCh. 15.3 - Prob. 15.1.4QCCh. 15.4 - Prob. 1CTQCh. 15.4 - Prob. 2CTQCh. 15.4 - Prob. 3CTQCh. 15.4 - Prob. 15.2.1QCCh. 15.4 - Prob. 15.2.2QCCh. 15.4 - Prob. 15.2.3QC
Ch. 15 - Prob. 1ECh. 15 - Which organ synthesizes...Ch. 15 - Which of the following classes of leukocytes...Ch. 15 - Which class of leukocytes develops into...Ch. 15 - Prob. 5ECh. 15 - Prob. 6ECh. 15 - Prob. 7ECh. 15 - Prob. 8ECh. 15 - Describe the life cycle of erythrocytes. Be sure...Ch. 15 - Prob. 10ECh. 15 - Prob. 11ECh. 15 - Prob. 12E
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