Biological Science
Biological Science
5th Edition
ISBN: 9780321743671
Author: Scott Freeman
Publisher: PEARSON
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Chapter 15, Problem 3TYK
Summary Introduction

To review:

The given blank space in the statement, “The enzyme that removes twists of the DNA ahead of the replication fork is _________”.

Introduction:

Replication of DNA (deoxyribonucleic acid) is a process in which one of the strands of DNA gets duplicated resulting in the formation of another copy of the strand. This biological process is responsible for the inheritance of characters from one organism to their offspring and hence occurs in all the organisms existing on this earth.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.
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